Comparison of serum high-molecular weight (HMS adiponectin with total adiponectin concentrations in type 2 diabetic patients with coronary artery disease using a novel enzyme-linked immunosorbent assay to detect HMW adiponectin
Comparison of serum high-molecular weight (HMS adiponectin with total adiponectin concentrations in type 2 diabetic patients with coronary artery disease using a novel enzyme-linked immunosorbent assay to detect HMW adiponectin
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DOI：
10.2337/db05-1525
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发表时间：
2006-07-01
期刊：
影响因子：
7.7
通讯作者：
Nakano, Yasuko
中科院分区：
文献类型：
--
作者：
Aso, Yoshimasa;Yamamoto, Ruriko;Nakano, Yasuko
脂联素（Acrp 30）是一种脂肪细胞来源的蛋白质，以三聚体、六聚体和高分子量（HMW）形式存在于血清中，包括12-18个亚基。由于高分子量脂联素可能具有生物活性，我们使用一种新的酶联免疫吸附试验（ELISA）测定血清中的脂联素，并通过凝胶过滤色谱法证实，ELISA主要检测12-18亚基的脂联素，我们比较了2型糖尿病患者中高分子量脂联素与总脂联素的浓度。我们接下来研究了280例连续2型糖尿病患者血清HMW与冠状动脉疾病（CAD）之间的关系，其中包括59例经血管造影证实的CAD患者。血清中总脂联素通过市售ELISA测定。与血清总脂联素一样，HMW脂联素与HDL胆固醇呈正相关，与甘油三酯、胰岛素敏感性、肌酐清除率和循环炎症标志物呈负相关。女性的总脂联素和高分子量脂联素显著高于男性，高分子量脂联素与总脂联素的比值也显著高于男性。冠心病组血清HMW及HMW/脂联素比值显著低于非冠心病组（P < 0.05）。在女性中，当存在CAD时，该比率（但无论是总脂联素还是HMW脂联素）往往较低。总之，测定高分子量脂联素，特别是相对于血清总脂联素，对评估2型糖尿病患者的CAD是有用的。
Adiponectin (Acrp30), an adipocyte-derived protein, exists in serum as a trimer, a hexamer, and a high-molecular weight (HMW) form, including 12-18 subunits. Because HMW adiponectin may be biologically active, we measured it in serum using a novel enzyme-linked immunosorbent assay (ELISA) confirmed by gel filtration chromatography that the ELISA detected mainly adiponectin with 12-18 subunits, and we compared HMW with total adiponectin concentration in patients with type 2 diabetes. We next investigated the relationship between serum HMW and coronary artery disease (CAD) in 280 consecutive type 2 diabetic patients, including 59 patients with angiographically confirmed CAD. Total adiponectin was measured in serum by a commercially available ELISA. Like serum total adiponectin, HMW adiponectin correlated positively with HDL cholesterol and negatively with triglyceride, insulin sensitivity, creatinine clearance, and circulating inflammatory markers. Total and HMW adiponectin were significantly higher in women than in men, as was the HMW-to-total adiponectin ratio. Serum HMW and the HMW-to-total adiponectin ratio were significantly lower in men with than without CAD (P < 0.05, respectively). In women, the ratio, but neither total nor HMW adiponectin, tended to be lower when CAD was present. In conclusion, determination of HMW adiponectin, especially relative to total serum adiponectin, is useful for evaluating CAD in type 2 diabetic patients.