Real time quantitative PCR
Real time quantitative PCR
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DOI：
10.1101/gr.6.10.986
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发表时间：
1996-10-01
期刊：
影响因子：
7
通讯作者：
Williams, PM
中科院分区：
文献类型：
--
作者：
Heid, CA;Stevens, J;Williams, PM
我们已经开发了一种新的“实时”定量PCR方法。该方法通过双标记的荧光探针（即，TaqMan探针）。该方法提供了非常准确和可重复的基因拷贝定量。与其他定量PCR方法不同，实时PCR不需要PCR后样品处理，从而防止潜在的PCR产物遗留污染，并导致更快和更高的通量测定。实时PCR方法具有非常大的起始靶分子测定的动态范围（至少五个数量级）。实时定量PCR是非常准确的，比目前的定量PCR方法劳动强度低。
We have developed a novel ''real time'' quantitative PCR method. The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe). This method provides very accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much Faster and higher throughput assays. The real-time PCR method has a very large dynamic range of starting target molecule determination (at least five orders of magnitude). Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.