Multiscale quantification of morphodynamics: MorphoLeaf software for 2D shape analysis
Multiscale quantification of morphodynamics: MorphoLeaf software for 2D shape analysis
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DOI：
10.1242/dev.134619
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发表时间：
2016-09-15
期刊：
影响因子：
4.6
通讯作者：
Laufs, Patrick
中科院分区：
文献类型：
--
作者：
Biot, Eric;Cortizo, Millan;Laufs, Patrick
形态计量学的一个主要挑战是分析由不同尺度的结构形成的复杂生物形状。树叶就是这一挑战的例证，因为它们将整体形状的差异与边缘较小的形状变化结合在一起，从而形成叶状或牙齿，联合收割机。目前的方法基于轮廓或地标分析是成功的量化无论是整体叶片形状或叶缘解剖，但未能结合两者。在这里，我们提出了一个全面的战略和相关的免费平台，定量，多尺度分析不同架构的叶片形态。为此，生物学相关的地标自动提取和分层，并用于指导重建准确的平均轮廓，正确地代表全球和本地的功能。利用这种方法，我们建立了拟南芥不同等级叶片发育轨迹的定量框架，并追溯了叶片异胚性的起源。当应用于不同的突变形式，我们的方法可以有助于更好地了解基因功能，因为我们在这里显示的作用，CUC2在拟南芥叶锯齿。最后，我们通过分析手部形态来说明我们的工具具有更广泛的适用性。
A major challenge in morphometrics is to analyse complex biological shapes formed by structures at different scales. Leaves exemplify this challenge as they combine differences in their overall shape with smaller shape variations at their margin, leading to lobes or teeth. Current methods based on contour or on landmark analysis are successful in quantifying either overall leaf shape or leaf margin dissection, but fail in combining the two. Here, we present a comprehensive strategy and its associated freely available platform for the quantitative, multiscale analysis of the morphology of leaves with different architectures. For this, biologically relevant landmarks are automatically extracted and hierarchised, and used to guide the reconstruction of accurate average contours that properly represent both global and local features. Using this method, we establish a quantitative framework of the developmental trajectory of Arabidopsis leaves of different ranks and retrace the origin of leaf heteroblasty. When applied to different mutant forms, our method can contribute to a better understanding of gene function, as we show here for the role of CUC2 during Arabidopsis leaf serration. Finally, we illustrate the wider applicability of our tool by analysing hand morphometrics.