Lymphocyte-induced angiogenesis: a quantitative and sensitive assay of the graft-vs.-host reaction.
Lymphocyte-induced angiogenesis: a quantitative and sensitive assay of the graft-vs.-host reaction.
复制标题
淋巴细胞诱导的血管生成：移植物-VS.宿主反应的定量和敏感测定。
DOI：
10.1084/jem.141.5.1084
复制
发表时间：
1975-05-01
期刊：
影响因子：
--
通讯作者：
Auerbach R
中科院分区：
文献类型：
--
作者：
Sidky YA;Auerbach R
本文介绍了一种新的、灵敏的测定免疫活性淋巴细胞皮内给药对辐照未免疫小鼠皮肤的影响的方法。我们称之为淋巴细胞诱导的血管生成（LIA）的测定涉及由这些感受态细胞的作用诱导的新血管分支的计数。与先前描述的正常淋巴细胞转移反应的情况一样，LIA是移植物抗肿瘤的表现。寄主反应，如利用适当遗传组合的实验所示。该反应呈剂量依赖性，在2 × 10 ~（-6）-5 ~4 × 10 ~（-6）细胞的剂量范围内，诱导的血管数与注射的免疫活性细胞数相关。在这些剂量水平下，脾脏、淋巴结和氢化可的松抗性胸腺细胞有效;骨髓和胸腺细胞无效。裸鼠脾细胞不能诱导LIA，而丝裂霉素C和照射的淋巴细胞可以启动但不能维持该反应。淋巴细胞诱导的血管生成之间的关系已经被讨论，因为这些发现对迟发型超敏反应，炎症和血管病理学有影响。
A new and sensitive assay for the effect of intracutanous administration of immunocompentent lymphocytes into the skin of irradiated unimmunized mice is described. The assay, which we have termed lymphocyte-induced angiogenesis (LIA) involves enumeration of new vascular branches induced by the action of these competent cells. As is the case for the previously described normal lymphocyte transfer reaction, LIA is a manifestation of the graft-vs.-host reaction, as shown by experiments utilizing appropaiate genetic combinations. The reaction is dose-dependent, and within the dose range of 2 times 10 minus 5 -4 times 10-6 cells the mumber of vessels induced correlates with the mumber of immunocompetent cells injected. At these dose levels spleen, lumph node, and hydrocortisone-resistant thymocytes are effective; bone marrow and thymus cells are not. Spleen cells from nude mice are incapable of inducing LIA, while mitomycin-C and irradiated lymphocytes can initiate but not maintain the reaction. The relationship between lymphocyte-induced angiogenesis has been discussed as have the implications of these findings to delayed hypersensitivity, inflammation, and vascular pathology.