Complete cDNA sequence of the fourth component of murine complement.
Complete cDNA sequence of the fourth component of murine complement.
复制标题
鼠补体第四种成分的完整 cDNA 序列。
DOI：
10.1073/pnas.82.17.5895
复制
发表时间：
1985
影响因子：
11.1
通讯作者：
Ogata,RT
中科院分区：
文献类型：
--
作者：
Sepich,DS;Noonan,DJ;Ogata,RT
我们利用Okayama和Berg的方法构建了横跨小鼠品系B10.WR的小鼠补体第四组分（C4）整个长度的cDNA克隆。cDNA序列跨越5372个核苷酸，编码一个长度为1738个氨基酸的pre-pro-C4分子；它分别在C4 mRNA的5‘端和3’端包含56个和99个未翻译碱基。推断出的pre-pro-C4分子包括一个19个氨基酸的信号肽和两个高度碱性的链间区域，这些区域可能在蛋白质成熟过程中被切除。在蛋白质编码区长度上，核苷酸序列与人类C4 cDNA序列的同源性为79%。B10的比较。WR C4序列与小鼠菌株FM的C4和性别限制蛋白（Slp）的序列(i)表明B10的C4蛋白在溶血活性方面存在差异。WR和FM是由于远离C1裂解位点和内部硫酯位点的结构变化，并且（ii）提出了C4基因来自B10的可能性。WR与其相邻的Slp基因发生了遗传交换。
We have used the method of Okayama and Berg to construct cDNA clones that span the entire length of the fourth component of murine complement (C4) from mouse strain B10.WR. The cDNA sequence spans 5372 nucleotides and encodes a pre-pro-C4 molecule 1738 amino acids in length; it includes 56 and 99 untranslated bases at the 5' and 3' ends, respectively, of the C4 mRNA. The deduced pre-pro-C4 molecule includes a 19 amino acid signal peptide and two highly basic interchain regions that presumably are excised during maturation of the protein. The nucleotide sequence shows 79% identity with the human C4 cDNA sequence over the length of the protein-coding region. A comparison of the B10.WR C4 sequence with those of C4 and sex-limited protein (Slp) from mouse strain FM (i) suggests that the difference in hemolytic activity between the C4 proteins from B10.WR and FM is due to structural changes distant from both the C1 cleavage site and the internal thioester site and (ii) raises the possibility that the C4 gene from B10.WR has undergone genetic exchange with its adjoining Slp genes.