Receptive-field properties of rat ventral posterior medial neurons before and after selective kainic acid lesions of the trigeminal brain stem complex.
Receptive-field properties of rat ventral posterior medial neurons before and after selective kainic acid lesions of the trigeminal brain stem complex.
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三叉神经脑干复合体选择性红藻氨酸损伤前后大鼠腹侧后内侧神经元的感受野特性。
DOI：
10.1152/jn.1987.57.5.1577
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发表时间：
1987
影响因子：
2.5
通讯作者：
Killackey,HP
中科院分区：
文献类型：
--
作者：
Rhoades,RW;Belford,GR;Killackey,HP
在尿素麻醉大鼠的丘脑腹侧后内侧核（VPM）上记录单个神经元。其中6只完整，28只出现了三叉神经主核（PrV）的kainic酸（KA）损伤，9只出现了三叉神经内插亚核（SpVi）的类似损伤。4只动物出现PrV病变，随后，间隔1-3个月，将KA注射到SpVi中。特别注意对触须偏转敏感的神经元的接受场特征。在正常动物中，我们共记录了167个VPM神经元，其中85% （n = 142）对触须敏感。其余的VPM细胞被保护毛偏转（8.4%）、皮肤压痕（0.6%）或触须或保护毛偏转（1.8%）所激发。7个细胞（4.2%）无反应。VPM三叉神经表征的地形与Waite（59）先前报道的相似。完整大鼠触须敏感神经元普遍具有快速适应反应（84.5%），仅16.2%具有定向选择性。绝大多数（80.3%）的触须敏感细胞仅被一个晶须的偏转激活（1.2 +/- 0.5，平均+/- SD）；没有人会因为超过四个触须的偏转而兴奋。将KA注射到其他完整大鼠（n = 9）的SpVi中，对触须敏感的VPM神经元的接受野特征没有明显影响。向PrV中注射KA可显著改变VPM细胞的接受野特性。将KA注射到5只大鼠的PrV后，在0 - 10小时内对45个VPM神经元进行了记录。在这些细胞中，4.4%的细胞被颤振偏转兴奋，其余的细胞无反应。这些动物的SpVi和上丘的额外记录表明，神经毒素可能没有损害内插神经元及其轴突。对22只在PrV KA病变后存活1-6天的大鼠394个VPM细胞进行了记录。这些实验表明，在此期间，对周围刺激有反应的丘脑细胞数量有所增加。损伤后6天（4只动物），我们记录的73个VPM神经元中有52.8%被体感刺激兴奋。其中，89.5%是由一个或多个神秘触须的偏转引起的。在这些大鼠中，激活给定VPM细胞的须的平均数量为6.3 +/- 2.0 （SD）。在KA注射PrV后存活30-90天的5只大鼠的VPM进行了记录。（摘要删节为400字）
Single neurons were recorded from the ventral posteromedial thalamic nucleus (VPM) of urethan-anesthetized rats. Six of these animals were intact, 28 sustained kainic acid (KA) lesions of trigeminal nucleus principalis (PrV), and 9 received similar lesions of trigeminal subnucleus interpolaris (SpVi). Four animals sustained PrV lesions that were followed, at an interval of 1-3 mo, by KA injections into SpVi. Special attention was paid to the receptive-field characteristics of neurons that were sensitive to deflection of the mystacial vibrissae. In normal animals, we recorded a total of 167 VPM neurons, 85% (n = 142) of which were vibrissa sensitive. The remaining VPM cells were excited by either guard hair deflection (8.4%), indentation of the skin (0.6%), or deflection of either vibrissae or guard hairs (1.8%). Seven cells (4.2%) were unresponsive. The topography of the trigeminal representation in VPM was similar to that reported previously by Waite (59). Vibrissa-sensitive neurons in intact rats generally gave rapidly adapting responses (84.5%), and only 16.2% were directionally selective. The vast majority (80.3%) of the vibrissa-sensitive cells were activated by deflection of only one whisker (1.2 +/- 0.5, mean +/- SD); none were excited by deflection of more than four vibrissae. Injections of KA into SpVi of otherwise intact rats (n = 9) had no appreciable effect on the receptive-field characteristics of vibrissa-sensitive VPM neurons. Injections of KA into PrV markedly altered the receptive-field properties of VPM cells. Recordings were made from 45 VPM neurons over a period extending from 0 to 10 h after KA injections into PrV in five rats. Of these cells, 4.4% were excited by vibrissa deflection and the remainder were unresponsive. Additional recordings from SpVi and the superior colliculus of these same animals indicated that the neurotoxin probably did not damage interpolaris neurons or their axons. Recordings were made from 394 VPM cells in 22 rats that survived 1-6 days after KA lesions of PrV. These experiments demonstrated an increase in the number of thalamic cells that were responsive to peripheral stimulation over this period. By 6 days after the lesion (4 animals), 52.8% of the 73 VPM neurons we recorded were excited by somatosensory stimuli. Of these, 89.5% were activated by deflection of one or more mystacial vibrissae. The average number of whiskers that excited a given VPM cell in these rats was 6.3 +/- 2.0 (SD). Recordings were made from VPM in five rats that survived 30-90 days after KA injections in PrV.(ABSTRACT TRUNCATED AT 400 WORDS)