Adjuvant effect of Lactobacillus casei in a mouse model of gluten sensitivity
Adjuvant effect of Lactobacillus casei in a mouse model of gluten sensitivity
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DOI：
10.1016/j.imlet.2008.04.006
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发表时间：
2008-08-15
影响因子：
4.4
通讯作者：
Rossi, Mauro
中科院分区：
文献类型：
--
作者：
D'Arienzo, Rossana;Maurano, Francesco;Rossi, Mauro
据报道，益生菌菌株在肠道相关淋巴组织中发挥免疫调节活性。在这项研究中，我们探讨了干酪乳杆菌在转基因小鼠表达的人类DQ 8异源二聚体，HLA分子与乳糜泻（CD）。用谷蛋白组分麦醇溶蛋白粘膜免疫的DQ 8小鼠，如在CD中观察到的那样产生肠道Th1样应答，而不发生肠病。L.致敏小鼠中的酪蛋白特异性增强CD+ T细胞介导的麦胶蛋白特异性应答。值得注意的是，小肠粘膜中的麦胶蛋白特异性IFN γ表达的强烈增加和细胞因子环境的促炎极化都与益生菌菌株的存在相关。然而，这种情况没有引起任何粘膜改变。这些发现表明，麦胶蛋白特异性肠病不仅与HLA DQ 8限制的IFN γ的大量产生有关，还涉及其他参数。此外，我们的数据表明，L。干酪素可用于进一步增强粘膜和全身T细胞介导的应答。(C)2008 Elsevier B.V.保留所有权利。
Probiotic strains have been reported to exert immunomodulatory activities in the gut-associated lymphoid tissue. In this study we explored the effect of Lactobacillus casei in transgenic mice expressing the human DQ8 heterodimer, a HLA molecule linked to Celiac Disease (CD). DQ8 mice, mucosally immunized with the gluten component gliadin, mounted an intestinal Th1-like response as observed in CD, without developing enteropathy. Co-administration of L. casei in sensitized mice specifically enhanced the gliadin-specific response mediated by CD+ T cells. Notably, both a strong increase of the gliadin-specific IFN gamma expression and a pro-inflammatory polarization of the cytokine milieu in the small intestinal mucosa were associated to the presence of the probiotic strain. However, this condition did not bring on any mucosal alteration. These findings suggest that the gliadin-specific enteropathy is not merely related to the HLA DQ8-restricted massive production of IFN gamma, but additional parameters are involved. Moreover, our data imply that the intrinsic adjuvanticity of L. casei can be exploited to further enhance both mucosal and systemic T cell-mediated responses. (C) 2008 Elsevier B.V. All rights reserved.