Transcriptome Analysis of Entamoeba histolytica Trophozoites during in vivo Contact-independent Mediated Host-parasite Interaction: Putative Pathways Related to PCD
Transcriptome Analysis of Entamoeba histolytica Trophozoites during in vivo Contact-independent Mediated Host-parasite Interaction: Putative Pathways Related to PCD
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DOI：
10.9734/mrji/2022/v32i530390
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发表时间：
2022-08
期刊：
影响因子：
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通讯作者：
D. G. P. Ishiwara;M. Shibayama;K. Nakada-Tsukui;G. Jeelani;María Del Consuelo Gómez-García;Olivia Medel Flores;T. Nozaki
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文献类型：
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作者：
D. G. P. Ishiwara;M. Shibayama;K. Nakada-Tsukui;G. Jeelani;María Del Consuelo Gómez-García;Olivia Medel Flores;T. Nozaki
目的：在本研究中，我们利用DNA微阵列为基础的转录组分析，并显示了整体的变化，在体内的阿米巴滋养体与动物可溶性因子的相互作用，使用腹膜透析袋模型，以阐明假定的分子途径和基因参与这种相互作用。研究设计：我们利用基于DNA微阵列的转录组分析。结果如下：一个包括上调基因及其邻居相互作用的网络分析揭示了11个功能相关模块的存在。获得的六个模块与内质网（ER）功能有关，如降解，应激，蛋白酶体泛素化，磷酸化，脂质代谢和蛋白质分选。此外，主要的转录变化显示的寄生虫在相互作用的开始归因于响应主机防御。这些数据与以下观点一致，即生存所必需的基因的协调表达，例如蛋白质合成的增加、细胞骨架重排、囊泡运输和参与细胞死亡的基因，包括钙失衡和与蛋白质降解相关的ER信号（ERAD），是阿米巴滋养体与动物可溶性因子之间体内相互作用期间的整体景观，提示内质网应激是导致大肠杆菌细胞程序性死亡的主要途径之一。溶组织剂结论：目前的研究结果显示的全球转录变化的寄生虫在与宿主环境的相互作用的早期阶段，在腹膜植入表明，相当大比例的阿米巴滋养体的基因表达的协同变化是由于寄生虫的细胞死亡信号的响应，由于ER压力。对潜在分子机制的详细了解可能表明通过促进其死亡途径有效消除这种寄生虫。
Aim: In the present study, we exploited DNA microarray-based transcriptome analysis and showed overall changes in gene expression in vivo of amoebic trophozoites that interact with animal soluble factors using an intraperitoneal dialysis bag model to elucidate putative molecular pathways and genes involved in this interaction. Study Design: We exploited DNA microarray-based transcriptome analysis. Results: An analysis from a network including the interactions of up-regulated genes and their neighbors revealed the presence of 11 functionally related modules. Six of the modules obtained were related to endoplasmic reticulum (ER) functions, such as degradation, stress, proteasome-ubiquitination, phosphorylation, lipid metabolism, and protein sorting. Furthermore, major transcriptional changes displayed by the parasite at the beginning of interaction were attributed to the response to the host defense. These data are consistent with the notion that the concerted expression of genes necessary for survival such as increment in protein synthesis, cytoskeleton rearrangement, vesicular traffic and genes involved in cell death including calcium imbalance and the ER signals associated with protein degradation (ERAD) is an overall landscape during the in vivo interaction between the amoebic trophozoites and animal soluble factors, and suggest that the ER stress is one of the main pathways leading to programmed cell death in E. histolytica. Conclusion: The present findings on the global transcriptional changes displayed by the parasite at the early stages of interaction with host environments in peritoneal implantation indicate that a substantial proportion of concerted changes in gene expression in amoebic trophozoites are attributable to the parasite’s response for cell death signals due to ER stress. A detailed knowledge of the underlying molecular mechanism might suggest the efficient elimination of this parasite by promoting their death pathways.