Iron acquisition and oxidative stress response in aspergillus fumigatus.
Iron acquisition and oxidative stress response in aspergillus fumigatus.
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DOI：
10.1186/s12918-015-0163-1
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发表时间：
2015-04-24
影响因子：
--
通讯作者：
Laubenbacher R
中科院分区：
文献类型：
--
作者：
Brandon M;Howard B;Lawrence C;Laubenbacher R
Aspergillus fumigatus is a ubiquitous airborne fungal pathogen that presents a life-threatening health risk to individuals with weakened immune systems. A. fumigatus pathogenicity depends on its ability to acquire iron from the host and to resist host-generated oxidative stress. Gaining a deeper understanding of the molecular mechanisms governing A. fumigatus iron acquisition and oxidative stress response may ultimately help to improve the diagnosis and treatment of invasive aspergillus infections. This study follows a systems biology approach to investigate how adaptive behaviors emerge from molecular interactions underlying A. fumigatus iron regulation and oxidative stress response. We construct a Boolean network model from known interactions and simulate how changes in environmental iron and superoxide levels affect network dynamics. We propose rules for linking long term model behavior to qualitative estimates of cell growth and cell death. These rules are used to predict phenotypes of gene deletion strains. The model is validated on the basis of its ability to reproduce literature data not used in model generation. The model reproduces gene expression patterns in experimental time course data when A. fumigatus is switched from a low iron to a high iron environment. In addition, the model is able to accurately represent the phenotypes of many knockout strains under varying iron and superoxide conditions. Model simulations support the hypothesis that intracellular iron regulates A. fumigatus transcription factors, SreA and HapX, by a post-translational, rather than transcriptional, mechanism. Finally, the model predicts that blocking siderophore-mediated iron uptake reduces resistance to oxidative stress. This indicates that combined targeting of siderophore-mediated iron uptake and the oxidative stress response network may act synergistically to increase fungal cell killing. The online version of this article (doi:10.1186/s12918-015-0163-1) contains supplementary material, which is available to authorized users.
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影响因子：
4.9
作者：
Fluckinger, M;Haas, H;Redl, B
通讯作者：
Redl, B
DOI：
10.1007/978-1-4614-4726-9_3
发表时间：
2013-01-01
期刊：
HOT TOPICS IN INFECTION AND IMMUNITY IN CHILDREN IX
影响因子：
--
作者：
Henriet, Stefanie;Verweij, Paul E.;Warris, Adilia
通讯作者：
Warris, Adilia
影响因子：
3.1
作者：
Hissen, AHT;Chow, JMT;Moore, MM
通讯作者：
Moore, MM
DOI：
10.1002/wsbm.1273
发表时间：
2014-09-01
影响因子：
7.9
作者：
Albert, Reka;Thakar, Juilee
通讯作者：
Thakar, Juilee
影响因子：
3.1
作者：
Hissen, AHT;Wan, ANC;Moore, MM
通讯作者：
Moore, MM