Visualizing non infectious and infectious Anopheles gambiae blood feedings in naive and saliva-immunized mice.
Visualizing non infectious and infectious Anopheles gambiae blood feedings in naive and saliva-immunized mice.
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DOI：
10.1371/journal.pone.0050464
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Bain O
中科院分区：
文献类型：
--
作者：
Choumet V;Attout T;Chartier L;Khun H;Sautereau J;Robbe-Vincent A;Brey P;Huerre M;Bain O
冈比亚按蚊是疟疾和淋巴丝虫病的主要传播媒介。节肢动物与宿主在皮肤界面的相互作用是复杂的、动态的。我们使用了一种全局方法来描述吸血过程中蚊子（感染或未感染）与哺乳动物皮肤之间的相互作用。活体视频显微镜被用来表征几个功能在血液喂养。并观察了伯氏疟原虫子孢子在真皮内的沉积和移动。我们还使用组织学技术来分析感染和未感染的喂养对幼稚小鼠皮肤细胞反应的影响。在探测阶段，口器在皮肤内高度移动的。随着蚊子年龄的增长，探测时间增加，可能对病原体传播产生影响。通过毛细血管进食实现补充。唾液腺中子孢子的存在改变了蚊子的行为，感染的雌性比未感染的雌性更倾向于探测（86%对44%）。当蚊子从用唾液免疫的小鼠的血管中吸血时，观察到喙尖端周围的白色区域。苔藓喂养引起了急性炎症反应，在天真的小鼠，达到峰值后三个小时的叮咬。多核细胞和肥大细胞与唾液沉积有关。我们描述了第一个可视化的唾液在皮肤免疫组织化学（IHC）与针对唾液的抗体。唾液沉积物和子孢子被检测到在皮肤长达18小时后咬。这项研究中，我们可视化的探测和充血阶段的冈比亚按蚊血餐，提供了精确的信息，昆虫的行为作为其感染状态的函数和存在或不存在的抗唾液抗体。它还提供了洞察血液喂养和病原体传播的炎症反应的可能后果。
Anopheles gambiae is a major vector of malaria and lymphatic filariasis. The arthropod-host interactions occurring at the skin interface are complex and dynamic. We used a global approach to describe the interaction between the mosquito (infected or uninfected) and the skin of mammals during blood feeding. Intravital video microscopy was used to characterize several features during blood feeding. The deposition and movement of Plasmodium berghei sporozoites in the dermis were also observed. We also used histological techniques to analyze the impact of infected and uninfected feedings on the skin cell response in naive mice. The mouthparts were highly mobile within the skin during the probing phase. Probing time increased with mosquito age, with possible effects on pathogen transmission. Repletion was achieved by capillary feeding. The presence of sporozoites in the salivary glands modified the behavior of the mosquitoes, with infected females tending to probe more than uninfected females (86% versus 44%). A white area around the tip of the proboscis was observed when the mosquitoes fed on blood from the vessels of mice immunized with saliva. Mosquito feedings elicited an acute inflammatory response in naive mice that peaked three hours after the bite. Polynuclear and mast cells were associated with saliva deposits. We describe the first visualization of saliva in the skin by immunohistochemistry (IHC) with antibodies directed against saliva. Both saliva deposits and sporozoites were detected in the skin for up to 18 h after the bite. This study, in which we visualized the probing and engorgement phases of Anopheles gambiae blood meals, provides precise information about the behavior of the insect as a function of its infection status and the presence or absence of anti-saliva antibodies. It also provides insight into the possible consequences of the inflammatory reaction for blood feeding and pathogen transmission.
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影响因子：
2.1
作者：
Lardeux, F;Cheffort, J
通讯作者：
Cheffort, J
影响因子：
15.3
作者：
Beghdadi, Walid;Porcherie, Adeline;Schneider, Bradley S.;Dubayle, David;Peronet, Roger;Huerre, Michel;Watanabe, Takeshi;Ohtsu, Hiroshi;Louis, Jacques;Mecheri, Salaheddine
通讯作者：
Mecheri, Salaheddine
影响因子：
3.1
作者：
Donovan, Michael J.;Messmore, Andrew S.;McDowell, Mary Ann
通讯作者：
McDowell, Mary Ann
影响因子：
6.7
作者：
Kebaier C;Voza T;Vanderberg J
通讯作者：
Vanderberg J
影响因子：
2.1
作者：
GARCIA, ES;MELLO, CB;RIBEIRO, JMC
通讯作者：
RIBEIRO, JMC