Extraction of cellular DNA from human cells and tissues fixed in ethanol.
Extraction of cellular DNA from human cells and tissues fixed in ethanol.
复制标题
从固定在乙醇中的人体细胞和组织中提取细胞 DNA。
DOI：
10.1016/0003-2697(87)90623-3
复制
发表时间：
1987
影响因子：
2.9
通讯作者：
Wakeland,EK
中科院分区：
文献类型：
--
作者：
Smith,LJ;Braylan,RC;Nutkis,JE;Edmundson,KB;Downing,JR;Wakeland,EK
DNA 可以从乙醇固定的淋巴细胞和组织中提取。固定程序简单、快速，DNA 提取本身与通常用于新鲜组织或细胞的提取相同。从乙醇固定材料中提取的 DNA 与从新鲜样品中提取的 DNA 的纯度、限制性内切酶消化的能力以及与 DNA 探针特异性杂交的能力没有区别。从乙醇固定的细胞和组织中提取 DNA 的能力消除了对新鲜或冷冻样本的严格处理和储存要求。
DNA can be extracted from ethanol-fixed lymphoid cells and tissues. The fixation procedure is simple and rapid, and the DNA extraction itself is the same as that normally used for fresh tissue or cells. DNA extracted from ethanol-fixed material is indistinguishable from DNA extracted from fresh samples based on its purity, its ability to be digested with restriction endonucleases, and its ability to specifically hybridize to DNA probes. The capability to extract DNA from ethanol-fixed cells and tissues eliminates the need for stringent handling and storage requirements of fresh or frozen specimens.