Increased Rho kinase activity in a Taiwanese population with metabolic syndrome
Increased Rho kinase activity in a Taiwanese population with metabolic syndrome
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DOI：
10.1016/j.jacc.2006.12.043
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发表时间：
2007-04-17
影响因子：
24
通讯作者：
Liao, James K.
中科院分区：
文献类型：
--
作者：
Liu, Ping-Yen;Chen, Jyh-Hong;Liao, James K.
目的探讨Rho激酶（ROCK）活性在代谢综合征（MetS）患者中的变化。背景近年来的研究表明，ROCK可能参与MetS的发病机制，但目前尚缺乏将ROCK与MetS联系起来的临床研究（60%男性，平均年龄55.5 ± 5.6岁），根据国家胆固醇教育计划成人治疗组III标准诊断为MetS，40名年龄和性别匹配的对照受试者。受试者的人口统计学进行了记录，血液samples were obtained.Results与对照组相比，ROCK活性，确定磷酸化的肌球蛋白结合亚基（MBS）在白细胞，是更大的MetS受试者（平均磷酸MBS/MBS比0.46与0.35，p = 0.002）。ROCK活性的截止值为0.39预测MetS的存在，特异性和敏感性为70%。血浆超敏C反应蛋白水平（5.5 mg/l，95%置信区间[CI] 3.1 - 7.2 mg/l vs. 2.8 mg/l，95% CI 1.1 - 3.9 mg/l，p = 0.01）和脂联素较低MetS受试者与对照受试者相比（4.9 μ g/ml，95%CI 3.2至6.1 μ g/ml vs. 5.9 μ g/ml，95%CI 4.2至7.5 μ g/ml，p = 0.01），但血浆白细胞介素-6和肿瘤坏死因子-α水平没有差异（两者p > 0.05）。体重指数、腰围、空腹血糖、高敏C反应蛋白和甘油三酯水平与ROCK活性水平升高相关。ROCK活性增加的风险随着MetS组分的数量增加而增加（p趋势< 0.001）。结论在台湾MetS受试者中Rho激酶活性增加，并且与MetS的每个组分和炎症标志物相关。这些结果表明，ROCK活性可能是一种新的MetS的血清学标志物。
Objectives We sought to determine whether Rho kinase (ROCK) activity is increased in a Taiwanese population with metabolic syndrome (MetS).Background Recent studies suggest that ROCK may be involved in the pathogenesis of MetS, but clinical studies linking ROCK with MetS are lacking.Methods We studied 40 Taiwanese subjects (60% men, mean age 55.5 +/- 5.6 years) who were diagnosed with MetS with National Cholesterol Educational Program Adult Treatment Panel III criteria and 40 age- and gender-matched control subjects. Subject demographics were recorded, and blood samples were obtained.Results Compared with control subjects, ROCK activity, as determined by phosphorylation of myosin binding subunit (MBS) in leukocytes, was greater in MetS subjects (mean phospho-MBS/MBS ratio 0.46 vs. 0.35, p = 0.002). A cutoff value for ROCK activity of 0.39 predicted the presence of MetS with specificity and sensitivity rates of 70%. Plasma high-sensitivity C-reactive protein was greater (5.5 mg/l, 95% confidence interval [CI] 3.1 to 7.2 mg/l vs. 2.8 mg/l, 95% Cl 1.1 to 3.9 mg/l, p = 0.01) and adiponectin was lower (4.9 mu g/ml, 95% Cl 3.2 to 6.1 mu g/ml vs. 5.9 mu g/ml, 95% Cl 4.2 to 7.5 mu g/ml, p = 0.01) in MetS subjects compared with control subjects, but plasma levels of interleukin-6 and tumor necrosis factor-alpha were not different (p > 0.05 for both). Body mass index, waist circumference, fasting glucose, high-sensitivity C-reactive protein, and triglyceride levels were associated with increased levels of ROCK activity. The risk of increased ROCK activity increased with the number of MetS components (p for trend < 0.001).Conclusions Rho kinase activity is increased in Taiwanese subjects with MetS and is associated with each component of MetS and markers of inflammation. These findings suggest that ROCK activity may be a novel serological marker of MetS.