Manipulation of living cells with 450 nm laser photobiomodulation
Manipulation of living cells with 450 nm laser photobiomodulation
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使用 450 nm 激光光生物调节操纵活细胞
DOI：
10.1016/j.jphotobiol.2020.111896
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Fu Xiaobing
中科院分区：
文献类型：
--
作者：
Hu Wenzhi;Zhang Yuehou;Li Bingmin;Li Qiankun;Ma Kui;Zhang Cuiping;Fu Xiaobing
越来越多的研究表明，光生物调节（PBM）影响细胞、组织和器官的特定生物学效应，这些效应依赖于光辐射的产生。在这项研究中，我们的目的是精确地操纵粘附细胞的空间排列在传统的培养条件下，与450 nm的低强度激光。经450nm激光照射后，培养细胞的粘附能力明显增强，并能抵抗0.1%胰蛋白酶的消化。结合计算机辅助设计系统（CAD）和计算机数控系统（CNC），设计的激光照射模式在培养皿中诱导特定的细胞微图案。RNA测序和生物化学实验证实，450 nm激光促使低密度脂蛋白（LDL）结合到细胞表面，并诱导脂质过氧化，交联和修饰照射细胞表面的蛋白质分子。以这种方式，过氧化产物修饰的蛋白质抵抗胰蛋白酶的蛋白水解，最终导致在胰蛋白酶处理下照射的和未照射的细胞之间的差异分离。这种方便的方法不需要特殊的生物材料处理，对细胞活力和功能没有影响，并且不需要改变常规的细胞培养条件。光诱导细胞捕获通过提供空间分辨率是对现有工具的极大补充。
Increasing studies demonstrated that photobiomodulation (PBM) influenced specific biological effects in cells, tissues and organs, and these effects rely on the production of light irradiation. In this study, we aimed to precisely manipulate the spatial arrangement of adhesion cells in a traditional culture condition with 450 nm low intensity laser. Through 450 nm laser PBM, the adhesion of the cultured cells was significantly improved and resisted the digestion of 0.1% trypsin. Combined with a computer aided design system (CAD) and computer numerical control (CNC) system, the designed laser irradiation pattern induced the specific cell micropattern in the culture dish. RNA sequencing and biochemical experiments confirmed that the 450 nm laser prompted low-density lipoprotein (LDL) bonding to the cell surface and induced lipid peroxidation, which crosslinked and modified the protein molecules on the irradiated cell surface. In this way, the peroxidation product-modified proteins resisted trypsin proteolysis, ultimately leading to a differential detachment between the irradiated and non-irradiated cells under trypsin treatment. This convenient method did not require special biomaterial processing, has no impact on cell viability and functions, and required no changes to the conventional cell culture conditions. The photo-induced cell capturing is a great complement to existing tools by providing spatial resolution.