Cryogenic 3D printed hydrogel scaffolds loading exosomes accelerate diabetic wound healing
Cryogenic 3D printed hydrogel scaffolds loading exosomes accelerate diabetic wound healing
复制标题
加载外泌体的低温3D打印水凝胶支架可加速糖尿病伤口愈合
DOI：
10.1016/j.cej.2021.130634
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发表时间：
2021-06-17
影响因子：
15.1
通讯作者：
Liu, Guohui
中科院分区：
文献类型：
--
作者：
Hu, Yiqiang;Wu, Bin;Liu, Guohui
糖尿病性伤口是影响患者健康和生活质量的一个紧迫问题。尽管治疗策略不断改进，糖尿病伤口仍然是一个全球性的挑战。血管功能障碍，部分是由于高血糖，是导致糖尿病伤口愈合不足的一个很好的因素。在目前的研究中，我们利用基于挤压的低温3D打印技术构建脱细胞小肠粘膜下层（SIS），结合介孔生物活性玻璃（MBG）和外泌体，制造出一种3D支架绷带（SIS/MBG@Exos），允许生物活性外泌体的持续释放。SIS/MBG@Exos水凝胶支架具有良好的三维结构，具有合适的孔隙度、生物相容性和止血能力，可促进人脐静脉内皮细胞（HUVECs）的增殖、迁移和血管生成。糖尿病创面体内实验结果表明，SIS/MBG@Exos水凝胶支架通过增加创面血流量，刺激糖尿病创面血管生成过程，加速糖尿病创面愈合。SIS/MBG@Exos水凝胶支架还能促进肉芽组织的形成，组织良好的胶原纤维沉积，功能性新血管的生长，促进伤口愈合的因素。综上所述，本研究为糖尿病伤口的治疗提供了一个有希望的新策略。
Diabetic wounds represent a pressing concern affecting the health and quality of life of patients. Despite the continuous improvement in therapeutic strategy, diabetic wounds remain a worldwide challenge. Vascular dysfunction, in part due to hyperglycemia, is a well-identified factor contributing to inadequate healing in diabetic wounds. In the current study, we utilize an extrusion-based cryogenic 3D printing technology to construct decellularized small intestinal submucosa (SIS) combined with mesoporous bioactive glass (MBG) and exosomes to fabricate a produce a 3D scaffold dressing (SIS/MBG@Exos) which permits sustained release of bioactive exosomes. The SIS/MBG@Exos hydrogel scaffolds possess a good 3D structure with an suitable porosity, biocompatibility and hemostasis ability, which could promote the proliferation, migration and angiogenesis of Human umbilical vein endothelial cells (HUVECs). The results of diabetic wounds in vivo indicate that the SIS/MBG@Exos hydrogel scaffolds accelerate diabetic wound healing through increasing the blood flow of wounds and stimulating the angiogenesis process of the diabetic wound. The SIS/MBG@Exos hydrogel scaffolds also promote granulation tissue formation, well-organized collagen fiber deposition, functional new blood vessel growth, factors promoting wound healing. Taken together, this research presents a promising novel strategy for the treatment of diabetic wounds.