CLASS-II ANTIGEN EXPRESSING CELLS IN EXPERIMENTALLY INDUCED PULPITIS
CLASS-II ANTIGEN EXPRESSING CELLS IN EXPERIMENTALLY INDUCED PULPITIS
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DOI：
10.1111/j.1365-2591.1991.tb00864.x
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发表时间：
1991-01-01
影响因子：
5
通讯作者：
JONTELL, M
中科院分区：
文献类型：
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作者：
BERGENHOLTZ, G;NAGAOKA, S;JONTELL, M
本研究报告了在实验诱导的大鼠切牙牙髓炎症病变中出现II类抗原表达细胞的情况。将刀豆蛋白A和牙周炎杆菌脂多糖通过牙槽骨和牙根中部的牙周组织涂抹于裸露的牙髓中。在一组对照牙齿中，牙髓暴露用Cavit覆盖，而不放置抗原性材料。分别在3、12、24、48或96小时后处死动物。牙髓组织标本用鼠抗鼠II类抗原单抗进行免疫组织化学分析。光镜下对阳性细胞进行半定量评价。在所有实验期间，攻击组牙髓中表达II类抗原的细胞明显多于对照组。细胞数量的增加在48小时时达到峰值，在随后的96小时观察中逐渐减少。II类抗原表达细胞的快速而强烈的涌入表明，这些细胞与牙髓的最初防御有关。
This study reports on the occurrence of class II antigen expressing cells in inflammatory lesions experimentally induced in the rat incisor pulp. Concanavalin A and lipopolysaccharide of Bacteroides gingivalis were applied to the exposed pulp following a preparation through alveolar bone and dental tissues in the midpart of the root. In a set of control teeth, pulpal exposures were capped with Cavit without the placement of antigenic material. Animals were killed after 3, 12, 24, 48 or 96 hours. Pulp tissue specimens were subjected to immunohistochemical analysis utilizing a monoclonal mouse anti-rat class II antigen antibody. Semi-quantitative assessment of positively stained cells was carried out under the light microscope. Significantly more class II antigen expressing cells were identified in the challenged pulps than in the controls at all experimental periods. The increase in cells peaked at 48 hours to taper off at the subsequent 96-hour observation. The rapid and intense influx of class II antigen expressing cells suggests that these cells are associated with the initial defence of the dental pulp.