THE DIARRHEAL RESPONSE OF HUMANS TO SOME CLASSIC SEROTYPES OF ENTEROPATHOGENIC ESCHERICHIA-COLI IS DEPENDENT ON A PLASMID ENCODING AN ENTEROADHESIVENESS FACTOR
THE DIARRHEAL RESPONSE OF HUMANS TO SOME CLASSIC SEROTYPES OF ENTEROPATHOGENIC ESCHERICHIA-COLI IS DEPENDENT ON A PLASMID ENCODING AN ENTEROADHESIVENESS FACTOR
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DOI：
10.1093/infdis/152.3.550
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发表时间：
1985-01-01
影响因子：
6.4
通讯作者：
OBRIEN, AD
中科院分区：
文献类型：
--
作者：
LEVINE, MM;NATARO, JP;OBRIEN, AD
Isolates of the most common O serogroups of enteropathogenic Escherichia coli (EPEC) associated with infant diarrhea (designated class I) adhere to Hep-2 cells; the genes for this adhesion, termed EPEC adherence factor (EAF), are located on plasmids 50-70 MDa in size. Volunteers ingested 1010 organisms of an O127:H6 Hep-2-adhesive class I strain (E2348/69) or its plasmid-minus, nonadhesive derivative. Diarrhea occurred in nine of 10 volunteers who ingested the patient strain (mean, 1,178 ml) but in only two of nine who took the plasmid-minus variant (mean, 433 ml; P < .006). All volunteers ill from strain E2348/69 mounted serum IgA and IgG responses to a 94-kDa plasmid-associated outer membrane protein of E2348/69; this protein was found in other class I EPEC but not in enterotoxigenic or meningitic strains. The 50-70-MDa EAF plasmid seems necessary for full expression of pathogenicity in EPEC that exhibit Hep-2 adhesiveness. EPEC isolates of certain other, less common, O serogroups (O44, O86, and O114) are rarely Hep-2 adhesive. These EPEC, designated class II, possess distinct 50-70 MDa plasmids lacking EAF genes. Diarrhea was caused by 108 or 1010 organisms of an 0114:H2 class II EPEC strain (mean, 1,156 ml) in six of 11 volunteers. This results confirmed that class II EPEC are pathogenic by a mechanism not involving Hep-2 adhesiveness.
