Structural mechanical properties of radiation-sterilized human Bone-Tendon-Bone grafts preserved by different methods.
Structural mechanical properties of radiation-sterilized human Bone-Tendon-Bone grafts preserved by different methods.
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DOI：
10.1007/s10561-015-9538-1
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发表时间：
2016-06
影响因子：
1.5
通讯作者：
Kamiński A
中科院分区：
文献类型：
--
作者：
Gut G;Marowska J;Jastrzebska A;Olender E;Kamiński A
为了避免传染病从供体传播给受体的风险，同种异体移植物应进行最终灭菌。在之前的论文（Kaminski 等人，细胞组织库 10:215–219）中，我们介绍了各种保存方法（深度新鲜冷冻、甘油化、冻干），然后用不同剂量的电子束 (EB) 照射，对在断裂拉伸试验中获得的用于前十字韧带重建的人髌腱切出的材料（内在）机械性能的影响。由于结构力学性能对于预测移植物作为一个整体功能单元的行为同样重要，因此本文的目的是展示在同一实验中获得的破坏载荷和伸长率的结果。成对的骨-肌腱-骨移植物（BTB）是由具有髌骨和胫骨附着物的尸体髌骨肌腱制备而成。它们通过深度冷冻、甘油化或冻干保存，随后用 25、35、50 或 100 kGy（新鲜冷冻移植物）或单剂量 35 kGy（甘油和冻干移植物）进行 EB 照射。每个实验（辐射）组均设有对照（未辐射）供体匹配组。使用Instron系统对所有组的样本进行机械破坏拉伸试验，以测量其结构性能（破坏载荷和伸长率）。所有冻干移植物在机械测试前均进行再水化。在我们的研究中，我们没有观察到通过新鲜冷冻处理然后使用剂量逐渐增加至 100 kGy 的 EB 进行最终灭菌的 BTB 移植物的结构机械性能显着恶化。相比之下，通过甘油化或冻干处理并用 35 kGy 辐照的 BTB 移植物显示出失效载荷显着降低。获得的结果表明，深度冷冻的辐照移植物在一定程度上保留了其初始机械性能，这并不排除其临床应用。然而，生物力学研究只是评估此类同种异体移植物潜在临床用途的第一步，还需要进一步广泛的体内研究。
To avoid the risk of infectious disease transmission from donor to recipient, allografts should be terminally sterilized. In the previous paper (Kaminski et al. in Cell Tissue Bank 10:215–219,) we presented the effect of various methods of preservation (deep fresh freezing, glycerolization, lyophilization), followed by irradiation with different doses of electron beam (EB), on material (intrinsic) mechanical properties of human patellar tendons cut out as for anterior cruciate ligament reconstruction, obtained in failure tensile test. As structural mechanical properties are equally important to predict the behaviour of the graft as a whole functional unit, the purpose of the present paper was to show the results for failure load and elongation, obtained in the same experiment. Paired Bone-Tendon-Bone grafts (BTB) were prepared from cadaveric human patella tendons with both patellar and tibial attachments. They were preserved by deep freezing, glycerolization or lyophilization and subsequently EB-irradiated with the doses of 25, 35, 50 or 100 kGy (fresh-frozen grafts) or a single dose of 35 kGy (glycerolized and lyophilized grafts). Each experimental (irradiated) group was provided with control (non-irradiated), donor-matched group. The specimens from all groups were subjected to mechanical failure tensile test with the use of Instron system in order to measure their structural properties (failure load and elongation). All lyophilized grafts were rehydrated before mechanical testing. In our study we did not observe significant deterioration of structural mechanical properties of BTB grafts processed by fresh-freezing and then terminal sterilized with growing doses of EB up to 100 kGy. In contrast, BTB grafts processed by glycerolization or lyophilization and irradiated with 35 kGy showed significant decrease of failure load. Obtained results suggest that deep-frozen irradiated grafts retain their initial mechanical properties to an extent which does not exclude their clinical application. However, biomechanical investigations constitute only the first step to evaluate the potential clinical usefulness of such allografts and further extensive in vivo studies are needed.