2 GLYCINE TRANSPORTER VARIANTS WITH DISTINCT LOCALIZATION IN THE CNS AND PERIPHERAL-TISSUES ARE ENCODED BY A COMMON GENE
2 GLYCINE TRANSPORTER VARIANTS WITH DISTINCT LOCALIZATION IN THE CNS AND PERIPHERAL-TISSUES ARE ENCODED BY A COMMON GENE
复制标题
DOI：
10.1016/0896-6273(93)90201-2
复制
发表时间：
1993-05-01
期刊：
影响因子：
16.2
通讯作者：
HOFFMAN, BJ
中科院分区：
文献类型：
--
作者：
BOROWSKY, B;MEZEY, E;HOFFMAN, BJ
我们分离了编码高亲和力、Na+/Cl-依赖性甘氨酸转运蛋白 GLYT-2 的 cDNA，它与另一种甘氨酸转运蛋白 GLYT-1 不同。虽然这两个 cDNA 的 3' 序列相同，但 5' 非编码区和 N 末端完全不同。 GLYT-1仅存在于中枢神经系统的白质中，而GLYT-2则存在于中枢神经系统的灰质以及周围组织的巨噬细胞和肥大细胞中。我们的研究结果表明，来自单个基因的组织特异性选择性剪接或选择性启动子使用会产生编码相似但不同甘氨酸转运蛋白的两种 mRNA 产物。 GLYT-2 mRNA 的解剖分布支持甘氨酸作为脊髓上神经递质的新兴地位，并表明甘氨酸可能充当 CNS 之外的化学信使。
We have isolated a cDNA encoding a high affinity, Na+/Cl--dependent glycine transporter, GLYT-2, which is distinct from another glycine transporter, GLYT-1. While the 3' sequences of these two cDNAs are identical, the 5' noncoding regions and the N-termini are completely different. GLYT-1 is found only in the white matter of the CNS, while GLYT-2 is found in the gray matter of the CNS as well as in macrophages and mast cells in peripheral tissues. Our findings suggest that tissue-specific alternative splicing or alternative promoter usage from a single gene results in two mRNA products encoding similar but distinct glycine transporters. The anatomic distibution of GLYT-2 mRNA supports the emerging status of glycine as a supraspinal neurotransmitter and suggests that glycine may function as a chemical messenger outside the CNS.