Impact of Collagen/Heparin Multilayers for Regulating Functions
Impact of Collagen/Heparin Multilayers for Regulating Functions
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DOI：
10.1021/acsami.6b09241
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发表时间：
2016-11-09
影响因子：
9.5
通讯作者：
Birch, Mark A.
中科院分区：
文献类型：
--
作者：
Ferreira, Ana M.;Gentile, Piergiorgio;Birch, Mark A.
在设计用于骨再生的表面界面时，骨细胞与细胞外基质（ECM）微环境的相互作用至关重要。在这项工作中，将骨 ECM 的主要成分 I 型胶原 (Coll) 和糖胺聚糖 (hep) 的逐层薄膜组装在聚 (L-乳酸) (PLLA) 基底上，以评估生物大分子涂层对细胞活性的影响。 PLLA 的表面改性表明 hep/coll 多层在 10 个双层后是稳定的（通过接触角、红外光谱和形态分析证实）。这种简单的方法提供了关于肝素对 I 型胶原层次结构的影响的新信息：成骨细胞样 (MC3T3-E1) 和人骨髓源性间充质干细胞 (hMSC) 的组织和随后的细胞反应。有趣的是，沉积的肝素层数（1 或 10）似乎在胶原蛋白自组装成原纤维、稳定纤维胶原蛋白层方面发挥着重要作用；并可能影响 hMSC 的活性。
Bone cell interaction with extracellular matrix (ECM) microenvironment is of critical importance when engineering surface interfaces for bone regeneration. In this work layer-by-layer films of type I collagen (Coll), the major constituent of bone ECM, and heparin (hep), a glycosaminoglycan, were assembled on poly(L-lactic acid) (PLLA) substrates to evaluate the impact of the biomacromolecular coating on cell activity. The surface modification of PLLA demonstrated that the hep/coll multilayer is stable after 10 bilayers (confirmed by contact angle, infrared spectroscopy, and morphological-analysis). This simple approach provided novel information On the effect of heparin, on type I collagen hierarchical :organization and subsequent cell response of osteoblast-like (MC3T3-E1) and human bone marrow-derived mesenchymal stein cells (hMSCs). Interestingly, the number of deposited heparin layers (1 or 10) appeared to play an important role in the self-assembly of collagen into fibrils, stabilizing the fibrous collagen layer; and potentially impacting hMSCs activity.