Proteomic comparison between abdominal and thoracic aortic aneurysms
Proteomic comparison between abdominal and thoracic aortic aneurysms
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DOI：
10.3892/ijmm.2014.1627
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发表时间：
2014-04-01
影响因子：
5.4
通讯作者：
Oda, Teiji
中科院分区：
文献类型：
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作者：
Matsumoto, Ken-Ichi;Satoh, Kazumi;Oda, Teiji
腹主动脉瘤（AAA）和胸主动脉瘤（TAAs）的发病机制不同。在这项研究中，揭示其生化特性的差异，我们进行了定量蛋白质组学分析AAAs和TAAs与相邻的正常主动脉（NA）组织相比。蛋白质组学分析揭示了AAA中的176种非冗余差异表达蛋白质和TAA中的189种蛋白质，这些蛋白质在每个7个样本中至少5个样本中常见。在鉴定的蛋白质中，分别有55和68个蛋白质是AAAs和TAAs所特有的，而在AAAs和TAAs中鉴定出121个蛋白质。PANTHER对AAAs和TAAs中独特蛋白质的过度表达分析揭示了AAAs中凝血途径和TAAs中整合素信号通路的显著下调。另一方面，Genesis分析揭示了121种蛋白质中的58种蛋白质的不同表达模式。PANTHER过度表达分析表明，这些蛋白质在血液凝固和纤溶酶原激活级联反应中的表达在AAAs中降低，而在TAAs中与NA组织相比增加。另一方面，整合素信号通路中的蛋白表达在AAA中增加，而在TAA中与NA组织相比降低。因此，本研究中的数据表明，在AAAs和TAAs中显示差异表达模式的蛋白质可能参与AAAs和TAAs的不同发病机制。
The pathogenesis of abdominal aortic aneurysms (AAAs) and that of thoracic aortic aneurysms (TAAs) is distinct. In this study, to reveal the differences in their biochemical properties, we performed quantitative proteomic analysis of AAAs and TAAs compared with adjacent normal aorta (NA) tissues. The proteomic analysis revealed 176 non-redundant differentially expressed proteins in the AAAs and 189 proteins in the TAAs which were common in at least 5 samples within 7 samples of each. Among the identified proteins, 55 and 68 proteins were unique to the AAAs and TAAs, respectively, whereas 121 proteins were identified in both the AAAs and TAAs. PANTHER overrepresentation analysis of the unique proteins in the AAAs and TAAs revealed a significant downregulation of the blood coagulation pathway in the AAAs and that of the integrin signaling pathway in the TAAs. On the other hand, Genesis analysis revealed distinct expression patterns of 58 proteins among the 121 proteins. PANTHER overrepresentation analysis of these 58 proteins revealed that the expression of these proteins in the blood coagulation and the plasminogen activating cascade was decreased in the AAAs, whereas it was increased in the TAAs compared with the NA tissues. On the other hand, the protein expression in the integrin signaling pathway was increased in the AAAs, whereas it was decreased in the TAAs compared with the NA tissues. Thus, the data presented in this study indicate that the proteins that show differential expression patterns in AAAs and TAAs may be involved in the distinct pathogenesis of AAAs and TAAs.