Genome-wide analysis of gene expression in adult Anopheles gambiae
Genome-wide analysis of gene expression in adult Anopheles gambiae
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DOI：
10.1111/j.1365-2583.2006.00610.x
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发表时间：
2006-02-01
影响因子：
2.6
通讯作者：
James, AA
中科院分区：
文献类型：
--
作者：
Marinotti, O;Calvo, E;James, AA
通过基因组测序，冈比亚按蚊现已成为比较生物学、进化生物学和发育生物学基础研究的强大工具。这些研究产生的知识有望揭示新型病媒控制和病原体传播阻断策略的分子靶标。对成年雄性冈比亚按蚊和非吸血雌性冈比亚按蚊的基因表达谱进行比较表明，大约 22% 的基因表现出性别依赖性调节。吃血的雌性在进食后 3 小时内将大部分新陈代谢转变为血液消化和产卵，这不利于其他活动，例如飞行和对环境刺激的反应。基因表达的变化在吸血后的第一天、第二天和第三天最为明显，此时所有基因中多达 50% 的转录物积累表现出显着变化。产下第一批卵后（吸血后 72 至 96 小时），蚊子返回非性营养阶段，与 3 日龄非吸血雌性相似但不完全相同。吸血后 15 天，蚊子的衰老和/或营养状态通过大约 5% 的基因下调反映出来。在本贡献的范围内，不可能全面描述每个分析时间点调控的大量基因以及它们所涉及的每个生化途径或生物过程。因此，我们描述了在成年蚊子生命中表现出转录物积累主要差异的基因组。然而，已经提供了一个公开的可搜索数据库（http://www.angagepuci.bio.uci.edu/），以便感兴趣的研究人员可以根据他们的需要，根据基因的描述、功能或基因表达变异的水平对特定基因组进行详细分析。
With their genome sequenced, Anopheles gambiae mosquitoes now serve as a powerful tool for basic research in comparative, evolutionary and developmental biology. The knowledge generated by these studies is expected to reveal molecular targets for novel vector control and pathogen transmission blocking strategies. Comparisons of gene-expression profiles between adult male and nonblood-fed female Anopheles gambiae mosquitoes revealed that roughly 22% of the genes showed sex-dependent regulation. Blood-fed females switch the majority of their metabolism to blood digestion and egg formation within 3 h after the meal is ingested, in detriment to other activities such as flight and response to environment stimuli. Changes in gene expression are most evident during the first, second and third days after a blood meal, when as many as 50% of all genes showed significant variation in transcript accumulation. After laying the first cluster of eggs (between 72 and 96 h after the blood meal), mosquitoes return to a nongonotrophic stage, similar but not identical to that of 3-day-old nonblood-fed females. Ageing and/or the nutritional state of mosquitoes at 15 days after a blood meal is reflected by the down-regulation of similar to 5% of all genes. A full description of the large number of genes regulated at each analysed time point and each biochemical pathway or biological processes in which they are involved is not possible within the scope of this contribution. Therefore, we present descriptions of groups of genes displaying major differences in transcript accumulation during the adult mosquito life. However, a publicly available searchable database (http://www.angagepuci.bio.uci.edu/) has been made available so that detailed analyses of specific groups of genes based on their descriptions, functions or levels of gene expression variation can be performed by interested investigators according to their needs.