Molecular dynamics simulations reveal that AEDANS is an inert fluorescent probe for the study of membrane proteins
Molecular dynamics simulations reveal that AEDANS is an inert fluorescent probe for the study of membrane proteins
复制标题
分子动力学模拟表明 AEDANS 是一种用于膜蛋白研究的惰性荧光探针
DOI：
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
M. A. Hemminga
中科院分区：
文献类型：
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作者：
W. Vos;Ae Marieke;Schor Ae;A. Baumgaertner;Ae D Peter;T. Ae;M. A. Hemminga
计算机模拟进行了一些AEDANS标记的单半胱氨酸突变体的小参考膜蛋白，M13主要外壳蛋白，覆盖60%的其一级序列。M13主要外壳蛋白是一种单跨膜α螺旋膜蛋白，在N-末端具有相对较大的水暴露区域。在10 ns的分子动力学模拟中，我们分析了AEDANS标签和天然色氨酸的行为，它们在以前的FRET实验中被用作受体和供体。结果表明，AEDANS是一种相对惰性的环境探针，当附着在膜蛋白上时可以不受阻碍地穿过脂质膜。
Computer simulations were carried out of a number of AEDANS-labeled single cysteine mutants of a small reference membrane protein, M13 major coat protein, covering 60% of its primary sequence. M13 major coat protein is a single membrane-spanning, α-helical membrane protein with a relatively large water-exposed region in the N-terminus. In 10-ns molecular dynamics simulations, we analyze the behavior of the AEDANS label and the native tryptophan, which were used as acceptor and donor in previous FRET experiments. The results indicate that AEDANS is a relatively inert environmental probe that can move unhindered through the lipid membrane when attached to a membrane protein.
基于聚丙氨酸的肽不能在完全水合的磷脂双层中形成稳定的跨膜α螺旋。
影响因子：
2.9
作者：
Lewis,RN;Zhang,YP;Hodges,RS;Subczynski,WK;Kusumi,A;Flach,CR;Mendelsohn,R;McElhaney,RN
通讯作者：
McElhaney,RN