SURVIVAL OF HEPATITIS-A VIRUS ON HUMAN HANDS AND ITS TRANSFER ON CONTACT WITH ANIMATE AND INANIMATE SURFACES
SURVIVAL OF HEPATITIS-A VIRUS ON HUMAN HANDS AND ITS TRANSFER ON CONTACT WITH ANIMATE AND INANIMATE SURFACES
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DOI：
10.1128/jcm.30.4.757-763.1992
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发表时间：
1992-04-01
影响因子：
9.4
通讯作者：
SATTAR, SA
中科院分区：
文献类型：
--
作者：
MBITHI, JN;SPRINGTHORPE, VS;SATTAR, SA
甲型肝炎病毒（HAV；HM175 株）在五位志愿者手上的存活情况通过将 10μl 粪便悬浮病毒沉积在每个指垫上并在 0、20、60、120、180 和 240 分钟后洗脱接种物来确定。 0 分钟时从每个指垫回收的病毒量约为 6.0 x 10(4) PFU。 4 小时结束时，指腹上仍可检测到 16% 至 30% 的最初可恢复病毒。 将 HAV 接种物（10-mu-l；大约 1.0 x 10(4) PFU）放置在指垫或 1 厘米直径的金属盘上，用于确定在接近 0.2 kg/cm2 的压力下接触 10 秒后病毒转移到清洁表面的情况。 当接种物干燥20分钟时，病毒从指垫到指垫、指垫到磁盘、磁盘到指垫的转移范围为2,667至3,484 PFU，而干燥4小时后可转移0至50 PFU。 仅将接触压力从 0.2 kg/cm2 提高到 1.0 kg/cm2 就会导致病毒转移量增加约三倍。 将摩擦力（手指在接触 10 秒期间转动 10 圈半）与低压力和高压力相结合，可使病毒转移的 PFU 分别增加两倍和三倍。 研究发现，无论采用何种转移方式，压力和摩擦都会显着影响 HAV 转移（F = 33.98；P < 0.05）。 在转移模式和压力或摩擦之间没有观察到统计学上显着的相互作用。 这项定量研究的结果表明，人手可能在 HAV 的直接和间接传播中发挥着重要作用。
The survival of hepatitis A virus (HAV; strain HM175) on the hands of five volunteers was determined by depositing 10-mu-l of fecally suspended virus on each fingerpad and eluting the inoculum after 0, 20, 60, 120, 180, and 240 min. The amount of virus recovered from each fingerpad at 0 min was approximately 6.0 x 10(4) PFU. At the end of 4 h, 16 to 30% of the initially recoverable virus remained detectable on the fingerpads. HAV inocula (10-mu-l; approximately 1.0 x 10(4) PFU) placed on fingerpads or 1-cm-diameter metal disks were used to determine virus transfer to clean surfaces upon a 10-s contact at a pressure of nearly 0.2 kg/cm2. When the inoculum was dried for 20 min, virus transfer from fingerpad to fingerpad, fingerpad to disk, and disk to fingerpad ranged from 2,667 to 3,484 PFU, while 0 to 50 PFU could be transferred after 4 h of drying. Elevation of the contact pressure alone from 0.2 to 1.0 kg/cm2 resulted in an approximately threefold increase in the amount of virus transferred. Incorporation of friction (10 half turns of the finger during 10 s of contact) with the low and high levels of pressure gave two- and threefold increases in the PFU of virus transferred, respectively. Pressure and friction were found to significantly affect HAV transfer (F = 33.98; P < 0.05), irrespective of the mode of transfer used. No statistically significant interaction was observed between mode of transfer and pressure or friction. The findings of this quantitative study suggest that human hands may play an important role in the direct as well as the indirect spread of HAV.