Relationship between phylogenetic groups, antibiotic resistance and patient characteristics in terms of adhesin genes in cystitis and pyelonephritis isolates of Escherichia coli
Relationship between phylogenetic groups, antibiotic resistance and patient characteristics in terms of adhesin genes in cystitis and pyelonephritis isolates of Escherichia coli
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DOI：
10.1016/j.micpath.2015.10.014
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发表时间：
2015-12-01
影响因子：
3.8
通讯作者：
Osmani, Agim
中科院分区：
文献类型：
--
作者：
Er, Doganhan Kadir;Dundar, Devrim;Osmani, Agim
肠外致病性大肠杆菌（E.大肠杆菌）被认为是全球泌尿道感染的主要病原体。抗生素耐药性、系统发生群、患者特征和粘附素毒力基因之间的关系是复杂的，尚未完全了解。本研究对146株尿中分离的E.用多重PCR方法检测了大肠杆菌、系统发育群和各种粘附素毒力基因。患者特征分为性别、膀胱炎和肾盂肾炎;社区获得性和医院获得性;复杂性和非复杂性感染。还测定了抗菌素耐药性。papAH基因在肾盂肾炎患者中的检出率高于膀胱炎患者，女性患者高于男性患者。iha基因在医院获得性感染中的频率高于社区获得性感染。sfa/focDE在氨苄西林、阿米卡星、庆大霉素、萘啶酸、诺氟沙星、头孢呋辛、头孢曲松、头孢唑啉、头孢噻肟、环丙沙星、甲氧苄啶/磺胺甲恶唑敏感和超广谱β-内酰胺酶（ESBL）及多药耐药（MDR）阴性菌株中较常见。focG多见于萘啶酸、诺氟沙星、头孢呋辛、头孢曲松、环丙沙星敏感和MDR阴性菌株。fimH和papAH分别在阿莫西林/克拉维酸和头孢噻肟敏感分离株中更常见。iha和afa/draBC基因在耐药菌株中的频率高于敏感菌株; iha基因在氨苄西林、阿莫西林/克拉维酸、萘啶酸、头孢呋辛、头孢曲松耐药和ESBL及MDR阳性菌株中的频率高于敏感菌株; afa/draBC基因在头孢噻肟、头孢呋辛、环丙沙星、甲氧苄啶/磺胺甲恶唑耐药和ESBL及MDR阳性菌株中的频率高于敏感菌株。ST 131 E.大肠杆菌毒力基因型直接影响其耐药性。属于该克隆组的分离株具有MDR并且通常携带afa/draBC和iha基因。我们的研究结果可能提供新的见解之间的关系，发病机制，病人的特点和耐药性的E。coli UTI。(C)2015爱思唯尔有限公司版权所有。
Extraintestinal pathogenic Escherichia coli (E. coli) is considered as the main causative agent of urinary tract infections worldwide. The relationship between antimicrobial resistance, phylogenetic groups, patient characteristics and adhesin virulence genes are complex and not fully understood. In this study, among 146 urinary isolates of E. coli, phylogenetic groups and various adhesin virulence genes were examined with multiplex Polymerase Chain Reaction methods. Patient characteristics divided into sex, cystitis and pyelonephritis; community-acquired and hospital-acquired; complicated and uncomplicated infection. Antimicrobial resistance was also determined. The papAH gene was seen more often in pyelonephritis than cystitis and female than male patients. iha gene was more frequent in hospital-acquired infections than in community-acquired infections. sfa/focDE was more frequent in ampicillin, amikacin, gentamicin, nalidixic acid, norfloxacin, cefuroxime, ceftriaxone, cefazolin, cefotaxime, ciprofloxacin and trimethoprim/sulfamethoxazole susceptible and extended-spectrum B-lactamase (ESBL) and multi-drug resistance (MDR) negative isolates. focG was seen more often in nalidixic acid, norfloxacin, cefuroxime, ceftriaxone, ciprofloxacin susceptible and MDR negative isolates. fimH and papAH were more commonly observed in amoxicillin/clavulanic acid and cefotaxime susceptible isolates, respectively. iha and afa/draBC genes were more frequent in resistant isolates than the susceptible ones; for iha, in ampicillin, amoxicillin/clavulanic acid, nalidixic acid, cefuroxime, ceftriaxone resistant and ESBL and MDR positive isolates; for afa/draBC, in cefotaxime, cefuroxime, ciprofloxacin, trimethoprim/sulfamethoxazole resistant and ESBL and MDR positive isolates, this trend was observed. ST 131 E. coli virulence gene pattern has a direct effect on resistance profile. Isolates belong to that clonal group has MDR and commonly harbour afa/draBC and iha genes. Our findings may provide new insights into the relationships between pathogenesis, patient characteristics and resistance of E. coli UTI. (C) 2015 Elsevier Ltd. All rights reserved.