LACK-specific CD4+ T cells that induce gamma interferon production in patients with localized cutaneous leishmaniasis during an early stage of infection
LACK-specific CD4+ T cells that induce gamma interferon production in patients with localized cutaneous leishmaniasis during an early stage of infection
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DOI：
10.1128/iai.70.6.3122-3129.2002
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发表时间：
2002-06-01
影响因子：
3.1
通讯作者：
Launois, P
中科院分区：
文献类型：
--
作者：
Bourreau, E;Prévot, G;Launois, P
在圭亚那利什曼原虫感染患者中，分析了可溶性利什曼原虫抗原（SLA）和利什曼原虫活化C激酶受体（LACK）的哺乳动物同源物（一种抗利什曼原虫病的候选疫苗）诱导的细胞因子谱，以及响应于这些抗原产生的细胞因子的细胞来源。γ干扰素（IFN-γ）和白细胞介素-10（IL-10）的产生是对LACK的反应。尽管仅在感染早期（感染开始后不到30天）分离出产生IFN-γ的LACK特异性CD 4（+）细胞，但在所有患者中均检测到产生IL-10的细胞。在所有患者中，产生IFN-γ和IL-13的CD 4（+）T细胞响应于SLA而产生。SLA-和LACK-特异性T细胞是效应记忆细胞，因为它们是CD 45 RA（-）CCR 7（-）CD 4（+）T细胞。产生IFN-γ的CD 4（+）T细胞为CD 62 L（-），产生IL-10的CD 4（+）T细胞为CD 62 L（+），表明这些细胞具有不同的组织归巢能力。这些发现表明SLA和LACK诱导1型（IFN-γ）和2型（IL-10或IL-13）细胞应答。
The profile of cytokines induced by soluble leishmania antigen (SLA) and the Leishmania homologue of the mammalian receptor for activated C kinase (LACK), a candidate vaccine against leishmaniasis, and the cellular source of the cytokines produced in response to these antigens were analyzed in patients infected with Leishmania guyanensis. Gamma interferon (IFN-gamma) and interleukin-10 (IL-10) were produced in response to LACK. Although LACK-specific CD4(+) cells producing IFN-gamma were isolated only during the early phase of infection (less than 30 days following the onset of infection), cells producing IL-10 in response to LACK were detected in all patients. CD4(+) T cells producing IFN-gamma and IL-13 were produced in response to SLA in all patients. SLA- and LACK-specific T cells are effector memory cells, as they are CD45RA(-) CCR7(-) CD4(+) T cells. CD4(+) T cells producing IFN-gamma are CD62L(-), and CD4(+) T cells producing IL-10 are CD62L(+), indicating that these cells have different tissue-homing capacities. These findings show that SLA and LACK induce both type 1 (IFN-gamma) and type 2 (IL-10 or IL-13) cell responses.