Analysis of the Continuous Bioconversion of Glycerol by Promotion of Highly Glycerol-Resistant Glycerol-Degrading Bacteria
Analysis of the Continuous Bioconversion of Glycerol by Promotion of Highly Glycerol-Resistant Glycerol-Degrading Bacteria
复制标题
通过高甘油抗性甘油降解细菌促进甘油的连续生物转化的分析
DOI：
10.1007/s12649-018-0344-4
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发表时间：
2018
影响因子：
3.2
通讯作者：
Tokumoto Hayato
中科院分区：
文献类型：
--
作者：
Kurahashi Kensuke;Hisada Keisuke;Kashiwagi Mai;Yoshihara Shizue;Nomura Toshiyuki;Tokumoto Hayato
目的通过添加葡萄糖对混合植物群进行调控，实现难降解甘油的连续生物转化。方法采用PCR结合变性梯度凝胶电泳（DGGE）技术，对添加葡萄糖后的混合植物群的16 S rRNA基因进行测序，研究其群落结构。结果越南伯克霍尔德氏菌（Burkholderia vietnamiensis）、苯酚鲁普氏伯克霍尔德氏菌（Burkholderia phenolirupestrium）、经鉴定，组成菌株为金黄色葡萄球菌、地衣芽孢杆菌和巴氏梭菌。利用殖民地.产氢率为0.34 mol/（mol甘油）. pasteurianum、巴氏杆菌B. licheniformis，B. B. vietnamiensis和B.苯酚三聚体利用甘油和葡萄糖作为底物，并且可以耐受高甘油负荷。在发酵早期，产氢菌C.巴氏戊二烯导致甘油转化为氢气和1，3-丙二醇。相反，在发酵后期，B.地衣和两个Burkholderiastrains使连续转化的甘油有价值的compounds.ConclusionsGlucose除了结果在一个稳定的植物群通过优化的比例，高度耐甘油的甘油降解细菌，从而建立一个厌氧消化过程，允许连续转化的高负荷的甘油。
PurposeWe identified component microorganisms in a fed-batch operation by modulating the mixed flora via addition of glucose to achieve continuous bioconversion of hardly degradable glycerol.MethodsTo study the microbial community structure of the flora accumulated by the addition of glucose, 16S ribosomal RNA (rRNA) gene was sequenced using PCR with denaturing gradient gel electrophoresis (DGGE).ResultsBurkholderia vietnamiensis, Burkholderia phenoliruptrix, Staphylococcus aureus, Bacillus licheniformis, andClostridium pasteurianumwere identified as component strains. Using the colony containingC. pasteurianum, the hydrogen yield was 0.34 mol/(mol glycerol).C. pasteurianum, B. licheniformis, B. vietnamiensis, andB. phenoliruptrixutilized both glycerol and glucose as substrates and could tolerate high glycerol loads. In early fermentation, predominance of the hydrogen-producingC. pasteurianumresulted in the conversion of glycerol into hydrogen and 1,3-propanediol. In contrast, in late fermentation, the auxiliary degradation ofB. licheniformisand the twoBurkholderiastrains enabled continuous conversion of the glycerol to valuable compounds.ConclusionsGlucose addition results in a stable flora by optimizing the ratio of highly glycerol-resistant glycerol-degrading bacteria, thereby establishing an anaerobic digestion process that allows continuous conversion of high loads of glycerol.