Reversible redox activity of ferrocene functionalized hydroxypropyl cellulose and its application to detect H2O2.
Reversible redox activity of ferrocene functionalized hydroxypropyl cellulose and its application to detect H2O2.
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DOI：
10.1016/j.carbpol.2015.11.077
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发表时间：
2016-04
影响因子：
11.2
通讯作者：
Pingping Li;Hongliang Kang;Chao Zhang;Weiwei Li;Yong Huang;Ruigang Liu
中科院分区：
文献类型：
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作者：
Pingping Li;Hongliang Kang;Chao Zhang;Weiwei Li;Yong Huang;Ruigang Liu
采用叠氮化物-炔环加成法制备了新型二茂铁功能化羟丙基纤维素（HPC-Fc），并对其进行了表征。HPC-Fc具有良好的可逆氧化还原活性，并能在酶和电极之间建立惊人的电子转移能力。将HPC-Fc和辣根过氧化物酶（HRP）包被在镀铂碳电极上，制备用于过氧化氢（H2O2）检测的安培生物传感器。在100 mM磷酸盐缓冲溶液（pH 7.0）中，测定了固定电位为0.35 V时h2o2浓度对电流响应的影响。该传感器在0.1 ~ 8 μM范围内对h2o2具有快速的线性响应，灵敏度为4.21 nA/μM。此外，分光光度计法测定的酶分析证实，HPC主干中丰富的羟基有助于HRP维持甚至增强其活性。具有氧化还原活性的HPC-Fc具有二茂铁和纤维素的独特性能，是生物传感器的良好候选材料。
Novel ferrocene functionalized hydroxypropyl cellulose (HPC-Fc) were prepared by azide-alkyne cycloaddition and characterized. HPC-Fc exhibits an excellent reversible redox activity and could establish amazing electron transfer ability between enzyme and electrode. HPC-Fc and horseradish peroxidase (HRP) were coated on a platinized carbon electrode to prepare an amperometric biosensor for hydrogen peroxide (H2O2) detection. The amperometric response was measured as a function of H2O2concentration at a fixed potential of 0.35 V in 100 mM phosphate buffer solution (pH 7.0). The novel biosensor exhibits a fast linear response toward H2O2in the range of 0.1–8 μM with sensitivity of 4.21 nA/μM. Moreover, the enzyme assays measured by the spectrophotometer method confirm that abundant hydroxyl groups of HPC backbones are conductive for HRP to maintaining or even enhancing their activity. The redox active HPC-Fc with the unique properties of both ferrocene and cellulose is a good candidate for biosensor applications.