Attenuating effect of casein glycomacropeptide on proliferation, differentiation, and lipid accumulation of in vitro Sprague-Dawley rat preadipocytes.
Attenuating effect of casein glycomacropeptide on proliferation, differentiation, and lipid accumulation of in vitro Sprague-Dawley rat preadipocytes.
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DOI：
10.3168/jds.2010-3827
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发表时间：
2011-02
影响因子：
3.5
通讯作者：
Siyu Xu;X. Mao;Fazheng Ren;H. Che
中科院分区：
文献类型：
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作者：
Siyu Xu;X. Mao;Fazheng Ren;H. Che
具有抑制前脂肪细胞增殖和细胞内脂质积累能力的食物成分可能有助于预防肥胖，这是一个全球性的健康问题。酪蛋白糖巨肽（GMP）具有显著的胃抑制活性，可能具有潜在的脂肪合成抑制特性和减轻肥胖的能力。本研究的目的是探讨GMP对前脂肪细胞的增殖和分化，以及从SD大鼠分离的前脂肪细胞的甘油三酯积累和甘油-3-磷酸脱氢酶活性的影响。将不同剂量（0、0.31、0.625、1.25、2.5和5.0 mg/mL）的GMP与前脂肪细胞共孵育。GMP处理组前体脂肪细胞的增殖活性明显低于对照组。GMP以剂量依赖性方式对前脂肪细胞增殖表现出抑制作用;在2.5 mg/mL时获得最大抗增殖作用。GMP也减弱分化，如脂质含量降低所揭示的，并且当细胞在分化诱导之前或开始时用GMP处理时，其效果比在细胞分化的后期阶段更明显。培养的前脂肪细胞处理GMP积累较少的甘油三酯和较低的甘油-3-磷酸脱氢酶活性比对照细胞没有GMP补充。结论：GMP在体外可抑制前脂肪细胞的增殖、分化和脂质积累。
Food components with the ability to suppress preadipocyte proliferation and intracellular lipid accumulation may be helpful in the prevention of obesity, which is a worldwide health concern. Casein glycomacropeptide (GMP), which has pronounced gastric inhibitory activity, could potentially possess fat synthesis inhibition properties and an obesity-alleviating capacity. The objective of the present study was to investigate the effect of GMP on the proliferation and differentiation of preadipocytes as well as triglyceride accumulation and glycerol-3-phosphate dehydrogenase activity in preadipocytes isolated from Sprague-Dawley rats. Different dosages (0, 0.31, 0.625, 1.25, 2.5, and 5.0 mg/mL) of GMP were co-incubated with preadipocytes. The proliferation activity of preadipocytes significantly decreased in the GMP-treated group compared with that of the control group without GMP supplementation. The GMP exhibited an inhibitory effect against preadipocyte proliferation in a dose-dependent manner; the maximal antiproliferative effect was obtained with 2.5 mg/mL. The GMP also attenuated differentiation, as revealed by decreased lipid content, and the effect was more pronounced when cells were treated with GMP before or at the beginning of differentiation induction than at later stages of cell differentiation. Cultured preadipocytes treated with GMP accumulated fewer triglycerides and had lower glycerol-3-phosphate dehydrogenase activity than did the control cells without GMP supplementation. In conclusion, GMP can inhibit the proliferation, differentiation, and lipid accumulation of preadipocytes in vitro.