Isolation, culture, and transplantation of intrahepatic biliary epithelial cells and oval cells.
Isolation, culture, and transplantation of intrahepatic biliary epithelial cells and oval cells.
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DOI：
10.1159/000163564
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
A. Sirica;G. Mathis;N. Sano;L. Elmore
中科院分区：
文献类型：
--
作者：
A. Sirica;G. Mathis;N. Sano;L. Elmore
最近在分离、培养和移植特定群体的肝内胆管上皮细胞和卵圆细胞方面取得的进展使得直接分析这些细胞类型在未转化或转化状态下的功能、生长特性和分化潜力成为可能。本文综述了从正常肝和胆汁淤积肝分离和培养肝内胆管上皮细胞，以及从癌前肝分离和培养卵圆细胞的各种方法。重点是比较这些不同类型的胆管细胞在体外的表型特征和生长特性，以及描述它们移植到异位组织部位的情况。此外，对卵圆细胞作为“兼性干细胞”在肝癌发生过程中的潜在作用进行了评估。
Recent advances made in the isolation, culture, and transplantation of defined populations of intrahepatic biliary epithelial cells and oval cells have permitted direct analysis of the functions, growth properties, and differentiation potential of these respective cell types in their untransformed or transformed states. This review provides a current and comprehensive examination of the various approaches that have been taken to isolate and culture intrahepatic biliary epithelial cells from normal and cholestatic liver and oval cells from preneoplastic liver. Emphasis is placed on comparing the phenotypic features and growth properties of these various biliary cell types in vitro as well as on describing their transplantation into ectopic tissue sites. In addition, the oval cell is evaluated in terms of its potential role as a 'facultative stem cell' during hepatocarcinogenesis.