SIMULTANEOUS DETERMINATION OF URINARY CREATININE AND METABOLITES OF TOLUENE, XYLENE, STYRENE, ETHYLBENZENE AND PHENOL BY AUTOMATED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
SIMULTANEOUS DETERMINATION OF URINARY CREATININE AND METABOLITES OF TOLUENE, XYLENE, STYRENE, ETHYLBENZENE AND PHENOL BY AUTOMATED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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DOI：
10.1007/bf00381617
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发表时间：
1988-01-01
影响因子：
3
通讯作者：
TAGUCHI, T
中科院分区：
文献类型：
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作者：
OGATA, M;TAGUCHI, T
建立了一种用自动高效液相色谱法直接测定尿中肌酐、马尿酸、甲基马尿酸和扁桃酸浓度的方法。用蒸馏水或移动的相稀释尿液，然后离心混合物，将上清液进样至HPLC中。使用填充有十八烷基硅烷化硅酸盐的不锈钢柱，并且移动的相是[含有3 mM 1-癸烷磺酸钠的20 mM磷酸一钾]/乙腈（85/15）的溶液。本文还介绍了另一种测定尿中苯酚、苯代谢物和/或苯酚浓度的HPLC方法。尿中的苯硫酸盐和苯基葡萄糖醛酸苷被酶水解为苯酚。将水解混合物进样到具有ODS柱的HPLC中。移动的相为[含有1 mM 1-癸烷磺酸钠的20 mM磷酸一钾]/乙腈（85/15）的溶液。运动后尿中马尿酸浓度与肌酐浓度的比值与运动前相似。此外，在车间的甲苯浓度和工人的尿中的HA浓度之间的相关系数，肌酐校正，高于甲苯浓度和未校正的HA浓度之间得到的。对于储存尿样的测定，将尿液点在滤纸上，干燥并保存数周，然后用50%甲醇洗脱滤纸中的MA、HA、o-MHA、m-MHA和肌酐，并通过HPLC测定其浓度。
An attempt was made to establish a method for the direct determination of urinary concentrations of creatinine, hippuric acid, methylhippuric acid and mandelic acid by automated high performance liquid chromatogrpahy (HPLC). Urine was diluted with distilled water or mobile phase, then the mixture was centrifuged and the supernatant was injected into HPLC. A stainless-steel column packed with octadecyl silanized silicate was used, and the mobile phase was a solution of [20 mM potassium phosphate monobasic containing 3 mM sodium 1-decanesulfonate]/acetonitorile (85/15). Another HPLC method for the determination of urinary concentration of phenol, metabolites of benzene and/or phenol is also described. Phenyl sulfate and phenyl glucuronide in urine were hydrolyzed enzymatically into phenol. The hydrolyzed mixture ws injected into HPLC with the ODS column. The mobile phase was a solution of [20 mM potassium phosphate monobasic containing 1 mM sodium 1-decanesulfonate]/acetonitorile (85/15). The ratio of hippuric acid (HA) concentration to creatinine concentration determined by the urine of students after physical exercise was similar to that before exercise. Moreover, the coefficient of correlation found between the toluene concentration in a workshop and the HA concentration in workers'' urine, corrected for creatinine, was higher than that obtained between the toluene concentration and the uncorrected HA concentration. For assays on stored urine samples, urine was spotted on filter paper, dried and kept several weeks, and then MA, HA, o-MHA, m-MHA and creatinine in the filter paper were eluted with 50% methanol and their concentrations determined by HPLC.