Differentiation, extracellular matrix synthesis, and integrin assembly by Drosophila embryo cells cultured on vitronectin and laminin substrates.
Differentiation, extracellular matrix synthesis, and integrin assembly by Drosophila embryo cells cultured on vitronectin and laminin substrates.
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在玻连蛋白和层粘连蛋白基质上培养的果蝇胚胎细胞的分化、细胞外基质合成和整合素组装。
DOI：
10.1002/aja.1001990205
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Fessler,JH
中科院分区：
文献类型：
--
作者：
Gullberg,D;Fessler,LI;Fessler,JH
两个对比的基板，Drosophilalaminin和人玻连蛋白，导致确定primaryDrosophilaembryo细胞遵循交替中间分化步骤，而不影响分化的最终结果。整合素αPS2β PS3对肌细胞在玻连蛋白上的初始铺展至关重要：富含β PS3整合素的局部接触形成，并通过含肌动蛋白和肌球蛋白的应力纤维连接。虽然α PS 2 β PS 3对于层粘连蛋白上的肌管形成是不必要的，但它对于随后的肌节细胞结构的改变是必需的。分化的原代培养物合成整合素并将其组装成不溶于去污剂的细胞骨架相关复合物。胶原IV、层粘连蛋白、肌动蛋白、乳头蛋白和其他细胞外基质蛋白主要由血细胞产生并分泌到培养基中。培养物内的进一步分化受到分泌成分和随后加入玻连蛋白或牛血清的影响。两种基质上的各种细胞类型的分化的比较表明，玻连蛋白提供了一个选择性的优势，肌细胞的分化，与上皮细胞，表皮细胞，和神经突富集。© 1994 Wiley‐利斯公司
Two contrasting substrates,Drosophilalaminin and human vitronectin, caused determined primaryDrosophilaembryo cells to follow alternate intermediate differentiation steps without affecting the final outcome of differentiation. Integrin αPS2βPS3was essential for the initial spreading of myocytes on vitronectin: focal contacts rich in βPS3integrins formed and were connected by actin‐ and myosin‐containing stress fibers. While αPS2βPS3was unnecessary for myotube formation on laminin, it was required for the subsequent change to a sarcomeric cytoarchitecture. The differentiating primary cultures synthesized integrins and assembled them into detergent‐insoluble, cytoskeleton‐associated complexes. Collagen IV, laminin, glutactin, papilin, and other other extracellular matrix proteins were made primarily by hemocytes and were secreted into the medium. Further differentiation within the cultures was influenced by secreted components and by later addition of vitronectin or bovine serum. Comparison of the differentiation of various cell types on the two substrates showed that vitronectin provided a selective advantage for the differentiation of myocytes, with enrichment over epithelia, epidermal cells, and neurites. © 1994 Wiley‐Liss, Inc.