Nonsynonymous site heteroplasmy in fish mitochondrial DNA
Nonsynonymous site heteroplasmy in fish mitochondrial DNA
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DOI：
10.1266/ggs.80.297
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发表时间：
2005-08-01
影响因子：
1.1
通讯作者：
Ida, H
中科院分区：
文献类型：
--
作者：
Shigenobu, Y;Saitoh, K;Ida, H
在野生动物线粒体DNA中很少观察到异质核苷酸多态。在非同义位点，由于功能限制，每个位点的核苷酸替换数量很少，这种位点异质性的发生被认为是极其罕见的。本文报道了大马哈鱼和比目鱼这两种野生鱼类的线粒体异质性。我们在鲑鱼NADH脱氢酶亚单位-5(ND5)区检测到一个与推测的氨基酸Ile或Met相对应的A/C非同义异质位点。异质位点位于第58密码子的第3位。对比目鱼，我们在ND4区检测到一个C/T非同义异质位点，对应于推测的氨基酸Leu或Pro。异质位点位于第450位密码子的第2位。我们还通过对克隆片段进行测序，验证了这些位点的异质性。
Heteroplasmic nucleotide polymorphisms are rarely observed in wild animal mitochondrial DNA. The occurrence of such site heteroplasmy is expected to be extremely rare at nonsynonymous sites where the number of nucleotide substitutions per site is low due to functional constraints. This report deals with nonsynonymous mitochondrial heteroplasmy from two wild fish species, chum salmon and Japanese flounder. We detected an A/C nonsynonymous heteroplasmic site corresponding to putative amino acids, Ile or Met, in NADH dehydrogenase subunit-5 (ND5) region of chum salmon. The heteroplasmic site was at the 3rd position of 58th codon. As for Japanese flounder we detected a C/T nonsynonymous heteroplasmic site corresponding to putative amino acids, Leu or Pro, in ND4 region. The heteroplasmic site was at the 2nd position of 450th codon. We also verified heteroplasmy at these sites by sequencing cloned fragments.