MEMBRANE TOPOLOGY OF THE 22-KDA INTEGRAL PEROXISOMAL MEMBRANE-PROTEIN
MEMBRANE TOPOLOGY OF THE 22-KDA INTEGRAL PEROXISOMAL MEMBRANE-PROTEIN
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DOI：
10.1016/0014-5793(93)81167-x
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发表时间：
1993-01-11
期刊：
影响因子：
3.5
通讯作者：
JUST, WW
中科院分区：
文献类型：
--
作者：
KALDI, K;DIESTELKOTTER, P;JUST, WW
为了在分子水平上研究大鼠肝脏22 kDa过氧化物酶体膜蛋白（PMP 22）的膜结构和可能的功能，我们从cDNA文库中克隆了PMP 22，并进行了序列测定。亲水性分析推断的一级结构表明4个推定的跨膜片段。PMP 22在完整过氧化物酶体中对外源性氨肽酶的可及性表明N-末端面向胞质溶胶。本文讨论了PMP 22在过氧化物酶体膜中的拓扑结构模型.同源性研究显示与Mpv 17基因产物惊人的相似性。缺乏这种膜蛋白会导致小鼠肾病综合征。
In order to study the membrane topology and the possible function of the rat liver 22 kDa integral peroxisomal membrane protein (PMP 22) at a molecular level, we have cloned PMP 22 from a lambdagt11 expression library and sequenced its cDNA. Hydropathy analysis of the deduced primary structure indicates 4 putative transmembrane segments. The accessibility to exogenous aminopeptidase of PMP 22 in intact peroxisomes suggests that the N-terminus faces the cytosol. A model of the topology of PMP 22 in the peroxisomal membrane is discussed. Homology studies revealed a striking similarity with the Mpv 17 gene product. Lack of this membrane protein causes nephrotic syndrome in mice.