Determination of the minimum fully protective dose of adenovirus-based DIVA vaccine against peste des petits ruminants virus challenge in East African goats.
Determination of the minimum fully protective dose of adenovirus-based DIVA vaccine against peste des petits ruminants virus challenge in East African goats.
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DOI：
10.1186/s13567-016-0306-4
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发表时间：
2016-01-21
影响因子：
4.4
通讯作者：
Baron MD
中科院分区：
文献类型：
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作者：
Holzer B;Taylor G;Rajko-Nenow P;Hodgson S;Okoth E;Herbert R;Toye P;Baron MD
小反刍兽疫病毒（PPRV）主要在发展中国家引起绵羊和山羊的经济上重要的疾病。它正成为国际社会加紧控制努力的对象。目前的疫苗不允许区分接种和感染的动物（没有DIVA能力）。我们以前已经表明，重组，复制缺陷型，腺病毒表达的PPRV H糖蛋白（AdH）提供了充分的保护野生型PPRV的挑战。我们现在已经测试了较低剂量的疫苗，以及AdH与表达PPRV F糖蛋白（AdF）的类似构建体的组合。我们在这里表明，在当地品种的山羊在一个国家，PPR疾病是常见的（肯尼亚），少至107 pfu的AdH提供显着的保护，对PPRV的挑战，而疫苗组成的108 pfu的AdH和AdF的每一个提供明显的无菌保护。这些发现强调了这些构建体作为DIVA疫苗用于PPR控制的效用。
Peste des petits ruminants virus (PPRV) causes an economically important disease of sheep and goats, primarily in developing countries. It is becoming the object of intensive international control efforts. Current vaccines do not allow vaccinated and infected animals to be distinguished (no DIVA capability). We have previously shown that recombinant, replication-defective, adenovirus expressing the PPRV H glycoprotein (AdH) gives full protection against wild type PPRV challenge. We have now tested lower doses of the vaccine, as well as AdH in combination with a similar construct expressing the PPRV F glycoprotein (AdF). We show here that, in a local breed of goat in a country where PPR disease is common (Kenya), as little as 107 pfu of AdH gives significant protection against PPRV challenge, while a vaccine consisting of 108 pfu of each of AdH and AdF gives apparently sterile protection. These findings underline the utility of these constructs as DIVA vaccines for use in PPR control.