Oncostatin M Induces Interleukin-6 and Cyclooxygenase-2 Expression in Human Vascular Smooth Muscle Cells
Oncostatin M Induces Interleukin-6 and Cyclooxygenase-2 Expression in Human Vascular Smooth Muscle Cells
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制瘤素 M 诱导人血管平滑肌细胞中白细胞介素 6 和环氧合酶 2 的表达
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
AlainTedgui
中科院分区：
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作者：
CatherineBernard;RégineMerval;MarilyneLebret;PhilippeDelerive;IsabelleDusanter;StéphanieLehoux;ChristopheCréminon;BartStaels;JacquesMaclouf;AlainTedgui
摘要：制瘤素 M (OSM) 是一种首先从活化的单核细胞和 T 淋巴细胞中鉴定出的细胞因子，是治疗艾滋病和卡波西肉瘤最有效的自分泌生长因子之一。关于 OSM 对正常血管细胞的影响知之甚少。因此，我们将人主动脉平滑肌细胞 (hASMC) 暴露于 OSM，检查细胞增殖和形态，并测定白细胞介素 6 (IL-6) 和环氧合酶 2 (COX-2) 的表达。 OSM具有弱的抗增殖作用。用 100 ng/mL OSM 孵育 4 天后，细胞计数减少至对照的 69±3%。然而，OSM 引起 hASMC 形态的显着变化，其特征是多克隆形状，与对照 hASMC 的纺锤体形态特征相反。 OSM 以剂量依赖性方式刺激 hASMC 释放 IL-6；暴露 48 小时后，OSM 浓度为 0、1、10 和 100 ng/mL 时，值分别为 8.5±0.7、29.7±3.5、50.9±4.4 和 73.8±7.6×103 U/mL (n=6)。 OSM 诱导 COX-2 蛋白和 mRNA 的显着表达。白血病...
Abstract—Oncostatin M (OSM), a cytokine first identified from activated monocytes and T lymphocytes, is one of the most potent autocrine growth factor for AIDS and Kaposi’s sarcoma. Little is known about the effects of OSM on normal vascular cells. We thus exposed human aortic smooth muscle cells (hASMCs) to OSM, examined cell proliferation and morphology, and determined interleukin-6 (IL-6) and cyclooxygenase-2 (COX-2) expression. OSM had a weak antiproliferative effect. After a 4-day incubation with 100 ng/mL OSM, cell count decreased to 69±3% of control. However, OSM induced striking changes in hASMC morphology, characterized by a polyclonal shape, in contrast to the spindle morphological feature of control hASMCs. OSM stimulated the release of IL-6 by hASMCs in a dose-dependent way; after a 48-hour exposure, values were 8.5±0.7, 29.7±3.5, 50.9±4.4, and 73.8±7.6×103 U/mL (n=6) at OSM concentrations of 0, 1, 10, and 100 ng/mL, respectively. OSM induced marked expression of COX-2 protein and mRNA. Leukem...