Molecular characterisation of potexviruses isolated from three different genera in the family Cactaceae
Molecular characterisation of potexviruses isolated from three different genera in the family Cactaceae
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DOI：
10.1007/s00705-003-0268-5
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发表时间：
2004-01
影响因子：
2.7
通讯作者：
R. Koenig;C. Pleij;S. Loss;W. Burgermeister;H. Aust;J. Schiemann
中科院分区：
文献类型：
--
作者：
R. Koenig;C. Pleij;S. Loss;W. Burgermeister;H. Aust;J. Schiemann
本文对分离自仙人掌科不同属的三种马铃薯X病毒的基因组特性进行了研究。对桥氏施氏霉（Schlumbergera bridgesii）分离物K11的基因组RNA的3′端3040个核苷酸进行了序列测定，并对仙人掌属（Zygocactussp.）分离物B1和CC10的RNA进行了全序列测定。和Opuntiasp.分别被确定。起始序列通过免疫捕获逆转录PCR使用衍生自其他马铃薯X病毒RNA中的高度保守序列的引物获得。序列的已知部分通过随机引物cDNA和衍生自序列的已知部分的特异性引物延伸。这三种病毒的基因组结构与其他马铃薯X病毒相似。在5个开放阅读框的翻译产物中容易地鉴定出马铃薯X病毒的复制相关蛋白、三重基因块（TGB）蛋白和外壳蛋白的典型保守基序。三种RNA的3′非翻译区折叠成含有三个特征性发夹的二级结构。外壳蛋白和TGB蛋白3的氨基酸序列同一性百分比相当低，分别为62%至76%和41%至49%，这表明这些病毒应被视为不同的病毒种，并建议将其命名为Zygocactus virus X、Schlumbergera virus X和Opuntia virus X。还建议不再使用最初为所有三种病毒分离物创造的名称仙人掌病毒X。
The genome properties of three potexviruses which previously had been isolated from different genera in the familyCactaceaeand had been found to be only distantly related serologically have been studied. The sequence of the 3040 3′ terminal nucleotides of the genomic RNA of isolate K11 fromSchlumbergera bridgesiiand the complete RNA sequences of isolates B1 and CC10 fromZygocactussp. andOpuntiasp., respectively, were determined. Starting sequences were obtained by means of immunocapture reverse transcription PCR using primers derived from highly conserved sequences in other potexviral RNAs. The known parts of the sequences were extended by means of random-primed cDNAs and specific primers derived from the known parts of the sequences. The genome structure of the three viruses resembles that of other potexviruses. The conserved motifs typical for replication-associated proteins, triple gene block (TGB) proteins and coat proteins of potexviruses were readily identified in the translation products of the five open reading frames. The 3′ untranslated regions of the three RNAs are folded into secondary structures containing three characteristic hairpins. Rather low percentages of amino acid sequence identities ranging from 62% to 76% for the coat proteins and 41% to 49% for TGB proteins 3 suggest that these viruses should be regarded as distinct virus species for which the names Zygocactus virus X, Schlumbergera virus X and Opuntia virus X are proposed. It is also suggested that the nameCactus virus Xwhich originally was coined for all three virus isolates should no longer be used.