A Potent Brucella abortus 2308 Δery Live Vaccine Allows for the Differentiation between Natural and Vaccinated Infection
A Potent Brucella abortus 2308 Δery Live Vaccine Allows for the Differentiation between Natural and Vaccinated Infection
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DOI：
10.1007/s12275-014-3689-9
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发表时间：
2014-08-01
影响因子：
3
通讯作者：
Zhang, Ke
中科院分区：
文献类型：
--
作者：
Zhang, Junbo;Yin, Shuanghong;Zhang, Ke
Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. However, the current Brucella abortus vaccines (S19 and RB51) are deficient; they can cause abortion in pregnant animals. Moreover, when the vaccine S19 is used, tests cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent vaccine is needed. A Brucella abortus 2308 ery promoter mutant (Delta ery) was constructed to overcome these drawbacks. The growth of the Delta ery mutant was significantly attenuated in macrophages and mice and induced high protective immunity in mice. Moreover, Delta ery induced an anti-Brucella-specific IgG (immunoglobulin G) response and stimulated the expression of interferon-gamma (INF-gamma) and interleukin-4 (IL-4). Furthermore, the expression of EryA antigen allowed for the serological differentiation between natural and vaccinated infection in mice. These results indicate that the Delta ery mutant is a potential attenuated live vaccine candidate against virulent Brucella abortus 2308 (S2308) infection.
