Quantitative Proteomic Analysis Reveals That Transmissible Gastroenteritis Virus Activates the JAK-STAT1 Signaling Pathway
Quantitative Proteomic Analysis Reveals That Transmissible Gastroenteritis Virus Activates the JAK-STAT1 Signaling Pathway
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定量蛋白质组学分析揭示传染性胃肠炎病毒激活 JAK-STAT1 信号通路
DOI：
10.1021/pr500173p
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发表时间：
2014-12-01
影响因子：
4.4
通讯作者：
Xiao, Shaobo
中科院分区：
文献类型：
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作者：
An, Kang;Fang, Liurong;Xiao, Shaobo
猪传染性胃肠炎病毒（TGEV）是一种致病性冠状病毒，可引起仔猪严重脱水和腹泻。本研究采用液相色谱-串联质谱联用同量异位素标记技术，对TGEV感染PK-15细胞后差异表达的蛋白质进行了定量分析。共鉴定了162个差异表达的细胞蛋白，包括60个上调蛋白和102个下调蛋白。这些差异表达的蛋白质参与细胞周期、细胞生长和增殖、先天免疫反应等。有趣的是，许多上调的蛋白质与干扰素信号传导相关，特别是信号转导和转录激活因子1（STAT 1）和干扰素刺激基因（ISG）。免疫印迹和实时定量逆转录聚合酶链反应表明，TGEV感染诱导STAT 1磷酸化和核转位，以及ISG表达。本研究首次从蛋白质组学角度揭示了TGEV诱导干扰素信号转导的机制。
Transmissible gastroenteritis virus (TGEV), a porcine enteropathogenic coronavirus, causes lethal watery diarrhea and severe dehydration in piglets. In this study, liquid chromatographytandem mass spectrometry coupled to isobaric tags for relative and absolute quantification labeling was used to quantitatively identify differentially expressed cellular proteins after TGEV infection in PK-15 cells. In total, 162 differentially expressed cellular proteins were identified, including 60 upregulated proteins and 102 downregulated proteins. These differentially expressed proteins were involved in the cell cycle, cellular growth and proliferation, the innate immune response, etc. Interestingly, many upregulated proteins were associated with interferon signaling, especially signal transducer and activator of transcription 1 (STAT1) and interferon-stimulated genes (ISGs). Immunoblotting and real-time quantitative reverse transcription polymerase chain reaction demonstrated that TGEV infection induces STAT1 phosphorylation and nuclear translocation, as well as ISG expression. This study for the first time reveals that TGEV induces interferon signaling from the point of proteomic analysis.