The chloroplast genome of the "basal" angiosperm Calycanthus fertilis -: structural and phylogenetic analyses
The chloroplast genome of the "basal" angiosperm Calycanthus fertilis -: structural and phylogenetic analyses
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DOI：
10.1007/s00606-003-0056-4
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发表时间：
2003-11-01
影响因子：
1.9
通讯作者：
Hellwig, FH
中科院分区：
文献类型：
--
作者：
Goremykin, V;Hirsch-Ernst, KI;Hellwig, FH
基础被子植物Calycanthusfertilis 的完整叶绿体基因组的核苷酸序列已被确定。环状 153337 bp 长的 cpDNA 与烟草、拟南芥和菠菜的 cpDNA 共线。基于与其他叶绿体基因组同源物的相似性，总共鉴定了 133 个预测基因（115 个单独的基因种类，18 个在反向重复中重复的基因），包括 88 个潜在的蛋白质编码基因（81 个基因种类）、8 个核糖体 RNA 基因（4 个基因种类）和 37 个 tRNA 基因（30 个基因种类），代表 20 个氨基酸。这是被子植物质体中记录的最高基因数量。 Calycanthus fertilis cpDNA 还包含最近发现的线粒体 ACRS 基因的同源物。由于没有记录从线粒体到叶绿体的基因转移，我们详细研究了该基因的进化亲和力。对质体蛋白编码子集的系统发育分析表明，古老的劳拉目系是在被子植物分裂成单子叶植物和双子叶植物之后出现的。
The nucleotide sequence of the complete chloroplast genome of a basal angiosperm, Calycanthusfertilis, has been determined. The circular 153337 bp long cpDNA is colinear with those of tobacco, Arabidopsis and spinach. A total of 133 predicted genes (115 individual gene species, 18 genes duplicated in the inverted repeats) including 88 potential protein-coding genes (81 gene species), 8 ribosomal RNA genes (4 gene species) and 37 tRNA genes (30 gene species) representing 20 amino acids were identified based on similarity to their homologs from other chloroplast genomes. This is the highest gene number ever registered in an angiosperm plastome. Calycanthus fertilis cpDNA also contains a homolog of the recently discovered mitochondrial ACRS gene. Since no gene transfer from mitochondria to the chloroplast has ever been documented, we investigated the evolutionary affinity of this gene in detail. Phylogenetic analysis of the protein-coding subset of the plastome suggests that the ancient line of Laurales emerged after the split of the angiosperms into monocots and dicots.