A mammalian cell mutant with a temperature-sensitive leucyl-transfer RNA synthetase.
A mammalian cell mutant with a temperature-sensitive leucyl-transfer RNA synthetase.
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具有温度敏感亮氨酰转移 RNA 合成酶的哺乳动物细胞突变体。
DOI：
10.1073/pnas.70.11.3094
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发表时间：
1973
影响因子：
11.1
通讯作者：
C. Stanners
中科院分区：
文献类型：
--
作者：
L. Thompson;J. Harkins;C. Stanners
中国仓鼠卵巢系的一个细胞突变体对蛋白质合成是温度敏感的，它在体内对tRNA充电亮氨酸的能力存在明显缺陷。胞质提取物具有温度敏感型亮氨酰-tRNA合成酶活性。因此，突变体很可能在亮氨酰-tRNA合成酶中发生了结构变化。该突变体的低泄漏和低返回率，再加上其蛋白质合成机制缺陷的特异性，使其成为研究动物细胞调节机制的一个有吸引力的工具。
A cell mutant of the Chinese hamster ovary line, which is temperature sensitive for protein synthesis, is specifically defective in vivo in its ability to charge tRNA with leucine. Cytoplasmic extracts exhibited temperature-sensitive leucyl-tRNA synthetase activity. It is, therefore, highly likely that the mutant has a structural alteration in leucyl-tRNA synthetase. The low leakiness and low reversion rate of this mutant, combined with the specificity of the defect in its protein-synthesizing machinery, make it an appealing tool for investigating regulatory mechanisms in animal cells.