Chitosan sponges as tissue engineering scaffolds for bone formation
Chitosan sponges as tissue engineering scaffolds for bone formation
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DOI：
10.1023/b:bile.0000032962.79531.fd
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发表时间：
2004-07-01
影响因子：
2.7
通讯作者：
Chung, CP
中科院分区：
文献类型：
--
作者：
Seol, YJ;Lee, JY;Chung, CP
用冷冻干燥法制备多孔壳聚糖海绵培养大鼠颅骨成骨细胞。制备的壳聚糖海绵具有孔径为100 ~ 200 μ m的多孔结构，有利于细胞增殖。细胞密度、碱性磷酸酶活性和钙沉积监测长达56 d的培养。细胞数量分别为每g海绵4 × 10(6)（第1天）、11 × 10(6)（第28天）和12 × 10(6)（第56天）。每块海绵的钙沉积量分别为9（第1天）、40（第28天）和48（第56天）杯。组织学结果证实海绵内的骨形成已经发生。结果表明，壳聚糖海绵可作为体外组织工程骨形成的有效支架材料。
Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100 - 200 mum pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 x 10(6) ( day 1), 11 x 10(6) ( day 28) and 12 x 10(6) (day 56) per g sponge. Calcium depositions were 9 ( day 1), 40 (day 28) and 48 (day 56) mug per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro.