Immunization, Isolation of Immunoglobulins and Antibody Titre Determination
Immunization, Isolation of Immunoglobulins and Antibody Titre Determination
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免疫、免疫球蛋白分离和抗体滴度测定
DOI：
10.1111/j.1365-3083.1983.tb04037.x
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发表时间：
1983
影响因子：
3.7
通讯作者：
A. Ingild
中科院分区：
文献类型：
--
作者：
N. Harboe;A. Ingild
在过去的10年中，我们研究了用多种免疫原免疫几千只远系繁殖兔，并分析了所得的抗血清。很明显，不存在在所有情况下都是最佳的并保证每只动物都有良好抗体应答的免疫程序。本章的目的是提到一些影响抗体应答的因素，并描述一种免疫方案，这种方案在统计学上有公平的机会产生良好的抗血清。我们还描述了一个简单的程序，用于分离的免疫球蛋白组分的兔抗血清和比较一些方法的抗体滴度测定和抗体特异性测定。整章的重点是大分子抗原的抗血清沉淀。
During the past 10 years we have studied the immunization of several thousand outbred rabbits with a multitude of immunogens and analysed the resulting antisera. It is obvious that an immunization procedure that is optimal in all situations and guarantees a good antibody response in every animal does not exist. The aim of this chapter is to mention some of the factors that influence the antibody response and to describe an immunization scheme that has a statistically fair chance of giving good antisera. We also describe a simple procedure for the isolation of the immunoglobulin fraction of rabbit antiserum and compare some methods for antibody titre determination and determination of antibody specificity. Throughout the chapter the emphasis is on precipitating antisera to macromolecular antigens.