The differential effects of cervical and thoracic dorsal funiculus lesions in rats
The differential effects of cervical and thoracic dorsal funiculus lesions in rats
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DOI：
10.1016/j.bbr.2007.09.035
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发表时间：
2008-03-05
影响因子：
2.7
通讯作者：
Muir, Gillian D.
中科院分区：
文献类型：
--
作者：
Kanagal, Srikanth G.;Muir, Gillian D.
本研究的目的是比较颈后脊索损伤的大鼠和胸中水平相同损伤的大鼠的运动能力。背索由上行感觉纤维和皮质脊髓束的主要成分组成，在脊髓C2或T8水平横断。我们检查了地面运动时四肢力量的产生、四肢的时机和协调性，以及在水平梯子上移动时的脚部放置错误。术后6周，双侧颈椎DF损伤导致地面运动时地面反作用力的产生和肢体计时发生细微但持续的变化，并导致梯子穿越时持续的前肢错误，而不是后肢错误。相反，同样位于胸中水平的损伤在损伤后2周不影响地面活动，仅造成轻微的前肢和后肢错误，到损伤后6周恢复到手术前水平。因此，颈部和胸部的DF损伤对大鼠的运动能力有不同的影响。我们将这些结果与以前的工作进行了比较，并认为DF横断术的不同反应可能与前肢和后肢之间的功能差异以及不同脊柱水平上背索的解剖差异有关。这些发现对恢复机制以及行为测试的类型都有影响，这些测试实际上可以用来测量不同损伤模型的功能变化。(C)2006爱思唯尔B.V.保留所有权利。
The purpose of this research was to compare the locomotor abilities of rats with cervical dorsal spinal funicular (DF) lesions to those of rats with the same lesion at the mid-thoracic level. The dorsal funiculus, consisting of ascending sensory fibers and the main component of the corticospinal tract, was transected either at spinal level C2 or at T8. We examined limb force generation and limb timing and coordination during overground locomotion, as well as foot placement errors during locomotion over a horizontal ladder. At 6 weeks post-surgery, bilateral lesions of the cervical DF caused subtle but persistent changes in the generation of ground reaction forces and limb timing during overground locomotion, and caused persistent forelimb, but not hindlimb, errors during ladder crossing. In contrast, the same lesion at the mid-thoracic level did not affect overground locomotion and caused only minor forelimb and hindlimb errors during ladder walking at 2 weeks post-lesion which recovered to pre-surgical levels by 6 weeks post-lesion. DF lesions at cervical vs. thoracic levels thus have differential effects on locomotor abilities in rats. We compare these results with previous work and suggest that the differential response to DF transection might be related to both functional distinctions between the fore- and hindlimbs and to anatomical differences in the dorsal funiculi at different spinal levels. These findings have implications for the mechanisms of recovery as well as the types of behavioural tests which can be practically used to measure functional changes in different lesion models. (C) 2006 Elsevier B.V. All rights reserved.