WHITE-OPAQUE TRANSITION - A 2ND HIGH-FREQUENCY SWITCHING-SYSTEM IN CANDIDA-ALBICANS
WHITE-OPAQUE TRANSITION - A 2ND HIGH-FREQUENCY SWITCHING-SYSTEM IN CANDIDA-ALBICANS
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DOI：
10.1128/jb.169.1.189-197.1987
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发表时间：
1987-01-01
影响因子：
3.2
通讯作者：
SOLL, DR
中科院分区：
文献类型：
--
作者：
SLUTSKY, B;STAEBELL, M;SOLL, DR
第二种高频开关系统在二态酵母菌白色念珠菌的选定致病菌株中被确定。在鉴定的菌株WO-1中，细胞以遗传的、可逆的、高频率（约0.1%）切换。在25度琼脂上形成的菌落的大小、形状和颜色容易区分的两种表型之间。c.在这个系统中，被称为“白-不透明过渡”，细胞形成“白色”半球形菌落，类似于白色念珠菌标准实验室菌株形成的菌落，或“不透明”菌落，更大，更平坦，灰色。WO-1还产生了至少三种其他可遗传集落表型，包括一种不规则皱型和两种模糊集落表型。白色不透明转变的基础似乎是细胞形态的根本差异。白细胞的形状、大小和出芽模式与实验室常见菌株相似。与之形成鲜明对比的是，不透明细胞呈豆状，其体积是白细胞的三倍，质量是白细胞的两倍，尽管这些可选择的表型在对数期含有相同数量的DNA和单个细胞核。除了形态上的差异外，白色和不透明细胞的产生时间、对低温和高温的敏感性以及形成菌丝的能力也存在差异。考虑了白光不透明过渡中高频开关的可能分子机制。
A second high-frequency switching system was identified in selected pathogenic strains in the dimorphic yeast Candida albicans. In the characterized strain WO-1, cells switched heritably, reversibly, and at a high frequency (.apprx. 10-2) between two phenotypes readily distinguishable by the size, shape, and color of colonies formed on agar at 25.degree. c. In this system, referred to as the "white-opaque transition," cells formed either "white" hemispherical colonies, which were similar to the ones formed by standard laboratory strains of C. albicans, or "opaque" colonies, which were larger, flatter, and grey. At least three other heritable colony phenotypes were generated by WO-1 and included one irregular-wrinkle and two fuzzy colony phenotypes. The basis of the white-opaque transition appears to be a fundamental difference in cellular morphology. White cells were similar in shape, size, and budding pattern of cells of common laboratory strains. In dramatic contrast, opaque cells were bean shaped and exhibited three times the volume and twice the mass of white cells, even though these alternative phenotypes contained the same amount of DNA and a single nucleus in the log phase. In addition to differences in morphology, white and opaque cells differed in their generation time, in their senstivity to low and high temperatures, and in their capacity to form hypae. The possible molecular mechanisms involved in high-frequency switching in the white-opaque transition are considered.