Dietary L-arginine supplementation enhances porcine β-defensins gene expression in some tissues of weaned pigs
Dietary L-arginine supplementation enhances porcine β-defensins gene expression in some tissues of weaned pigs
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DOI：
10.1016/j.livsci.2012.05.016
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发表时间：
2012-09-01
影响因子：
1.8
通讯作者：
Chen, Daiwen
中科院分区：
文献类型：
--
作者：
Mao, Xiangbing;Qi, Sharina;Chen, Daiwen
本研究旨在验证饲粮中添加L-精氨酸对断奶仔猪某些组织中β-防御素基因表达的调控作用。将30头21日龄断奶的杂交仔猪随机分为3组，每组10头，分别饲喂添加0.0%，0.5%，1.0%L-精氨酸的日粮。试验期为7 d。第7天，每组随机处死6头猪，采集组织和血样。与对照猪相比，饲粮中添加L-精氨酸不影响猪的生长性能和血清中伊加、IgG、IgM、IL-1 β、IL-2和INF-α的浓度（P> 0.05），但饲粮中添加0.5%的L-精氨酸可降低血清尿素浓度（P< 0.05）。此外，日粮中添加L-精氨酸显著增加了猪舌β-防御素-1基因、回肠和腹股沟淋巴结β-防御素-3基因以及口腔上皮、舌、回肠和腹股沟淋巴结β-防御素-2基因的表达（P
The present study was mainly conducted to test the hypothesis that dietary L-arginine supplementation regulates gene expression of beta-defensins in some tissues of weaned pigs. Thirty crossbred piglets weaned at 21 days of age were allotted to 1 of 3 diets supplemented with 0.0, 0.5 and 1.0% L-arginine (10 pigs/treatment). Pigs consumed the diets for 7 d. On d 7, 6 pigs from each treatment were randomly killed, and the tissue and blood samples were collected. Compared with control pigs, dietary L-arginine supplementation did not affect the growth performance and serum concentrations of IgA, IgG, IgM, IL-l beta, IL-2 and INF-alpha (P> 0.05), but dietary supplementation with 0.5% L-arginine reduced serum urea concentration (P< 0.05). Additionally, dietary L-arginine supplementation significantly increased porcine beta-defensin-1 gene expression of tongue, porcine beta-defensin-3 gene expression of ileum and inguinal lymph node, and porcine beta-defensin-2 gene expression of oral epithelium, tongue, ileum and inguinal lymph node (P