The tale of two RNA polymerases: transcription profiling and gene expression strategy of bacteriophage Xp10
The tale of two RNA polymerases: transcription profiling and gene expression strategy of bacteriophage Xp10
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DOI：
10.1111/j.1365-2958.2004.04442.x
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发表时间：
2005-02-01
影响因子：
3.6
通讯作者：
Severinov, K
中科院分区：
文献类型：
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作者：
Semenova, E;Djordjevic, M;Severinov, K
噬菌体Xp10感染水稻黄单胞菌的研究Xp10编码其自身的单亚基RNA聚合酶（RNAP），类似于T7家族的RNA聚合酶。另一方面，大多数Xp10基因的组织方式是典型的类胡萝卜素，已知它们的发育仅依赖于宿主RNAP。为了更好地了解Xp10发育过程中病毒转录的时间模式，我们对Xp10基因表达进行了全局转录谱分析、引物延伸、化学动力学建模和生物信息学分析。我们的研究结果表明，真正的镶嵌性质，Xp10依赖于宿主和病毒RNAP的表达基因编码的病毒体成分和宿主裂解。两种RNA聚合酶联合转录同一组基因对于噬菌体来说是前所未有的。奇怪的是，这种情况在叶绿体中也存在。
Bacteriophage Xp10 infects rice pathogen Xanthomonas oryzae. Xp10 encodes its own single-subunit RNA polymerase (RNAP), similar to that found in phages of the T7 family. On the other hand, most of Xp10 genes are organized in a manner typical of lambdoid phages that are known to rely only on host RNAP for their development. To better understand the temporal pattern of viral transcription during Xp10 development, we performed global transcription profiling, primer extension, chemical kinetic modelling and bioinformatic analyses of Xp10 gene expression. Our results indicate that true to its mosaic nature, Xp10 relies on both host and viral RNAPs for expression of genes coding for virion components and host lysis. The joint transcription of the same set of genes by two types of RNA polymerases is unprecedented for a bacteriophage. Curiously, such a situation is realized in chloroplasts.