EVALUATION OF DOUBLE THYMIDINE BLOCK FOR SYNCHRONIZING MAMMALIAN CELLS AT G1-S BORDER
EVALUATION OF DOUBLE THYMIDINE BLOCK FOR SYNCHRONIZING MAMMALIAN CELLS AT G1-S BORDER
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DOI：
10.1016/0014-4827(71)90599-4
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发表时间：
1971-01-01
影响因子：
3.7
通讯作者：
KIRKPATRICK, JB
中科院分区：
文献类型：
--
作者：
BOSTOCK, CJ;PRESCOTT, DM;KIRKPATRICK, JB
本文研究了双2 mM胸腺嘧啶核苷阻断对L细胞和CHOP细胞的影响。从第二个2 mM胸腺嘧啶核苷阻断剂释放后的S时相约为5h，缩短了约三分之一。未标记的有丝分裂在阻断剂释放后的第一个小时观察到，标记的有丝分裂在阻断剂5小时后观察到，在胸腺嘧啶核苷阻断剂的过程中，32P-正磷酸盐被掺入DNA中的速度大约是未阻断的对照细胞的三分之一。结果表明，在胸腺嘧啶核苷阻断期间，细胞继续以正常速率的三分之一合成DNA，并且不在G1-S交界处积聚。
The effect of a double 2 mM thymidine block on L cells and CHOP cells has been investigated. The S phase following release from the second 2 mM thymidine block is about 5 h which represents a shortening by about one-third. Unlabeled mitoses are observed in the first hours after release of the block and labeled mitoses are observed 5 h after the block,32P-orthophosphate is incorporated in the DNA during the thymidine block at about one-third the rate for non-blocked, control cells. The results indicate that cells continue to synthesize DNA during the thymidine block at about one-third the normal rate and do not accumulate at the G 1-S border.