Matrix Effects and Interferences of Different Citrus Fruit Coextractives in Pesticide Residue Analysis Using Ultrahigh-Performance Liquid Chromatography-High-Resolution Mass Spectrometry
Matrix Effects and Interferences of Different Citrus Fruit Coextractives in Pesticide Residue Analysis Using Ultrahigh-Performance Liquid Chromatography-High-Resolution Mass Spectrometry
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DOI：
10.1021/acs.jafc.7b00243
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发表时间：
2017-06-14
影响因子：
6.1
通讯作者：
Fernandez-Alba, Amadeo R.
中科院分区：
文献类型：
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作者：
Besil, Natalia;Cesio, Veronica;Fernandez-Alba, Amadeo R.
研究了7种不同柑橘果实的乙酸乙酯提取物对4GHz分辨率模式下液相色谱-高分辨率飞行时间质谱（UHPLC-（ESI）-HR-TOF）测定80种农药残留的基质影响。只有20%的评价农药表现出显着的基质效应（ME），由于与天然产物之间的共洗脱t（R）= 3和11分钟。主成分分析（PCA）检测到的共提取物分组的甜菊糖和橙品种，但分离柠檬，橙，甜菊糖。对方.基质效应在品种间存在差异，但在品种间存在相似性，这就要求对同一种水果进行农药残留量的测定时，必须采用基质匹配校正曲线。鉴定了23种天然产物（syneetin，柚苷，胭脂苷，橙皮苷，柠檬苦素，诺米林和一些脂肪酸等）。分析提取物。12个确定的化合物共洗脱与28个农药的研究，造成不同的基质效应。
The matrix effects of ethyl acetate extracts from seven-different citrus fruits on-the determination of 80 pesticide residues using liquid chromatography coupled to high-resolution time-of-flight mass spectrometry (UHPLC-(ESI)-HR-TOF) at 4 GHz resolution mode were studied. Only 20% of the evaluated pesticides Showed noticeable matrix effects (ME) due to coelution with natural products between t(R) = 3 and 11 min. Principal component analysis (PCA) of the detected coextractives grouped the mandarins and the-orange varieties, but separated lemon, oranges,and mandarins from. each other. Matrix effects were different among species but similar between varieties, forcing the determination of pesticide residues through matrix matched calibration curves with the same fruit. Twenty-three natural products (synephrine, naringin, poncirin, glycosides of hesperitin, limonin, nomilin, and a few fatty acids, among others) were identified in. the analyzed extracts. Twelve of the identified compounds coeluted with 28 of the pesticides under study, causing different matrix effects.