Sulfurisphaera javensis sp. nov., a hyperthermophilic and acidophilic archaeon isolated from Indonesian hot spring, and reclassification of Sulfolobus tokodaii Suzuki et al. 2002 as Sulfurisphaera tokodaii comb. nov.
Sulfurisphaera javensis sp. nov., a hyperthermophilic and acidophilic archaeon isolated from Indonesian hot spring, and reclassification of Sulfolobus tokodaii Suzuki et al. 2002 as Sulfurisphaera tokodaii comb. nov.
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DOI：
10.1099/ijsem.0.002765
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发表时间：
2018-04
影响因子：
2.8
通讯作者：
K. Tsuboi;H. Sakai;Naswandi Nur;K. Stedman;N. Kurosawa;A. Suwanto
中科院分区：
文献类型：
--
作者：
K. Tsuboi;H. Sakai;Naswandi Nur;K. Stedman;N. Kurosawa;A. Suwanto
从印度尼西亚的一个酸性温泉中分离到一种新的极端嗜热、嗜酸和兼性厌氧古菌菌株KD-1 T，其特征在于与产热相关的种大洼硫化叶菌Kurosawa et al.1998，Sulfolobus tokodaii Suzuki et al. 2002和阳明硫化叶菌（Sulfolobus yangmingensis Jan et al.1999）。KD-1 T细胞为不规则球菌，直径0.9-1.3 μm。该菌株在60-90 °C（最适80-85 °C）、pH 2.5-6.0（最适pH 3.5-4.0）和0- 1.0%（w/v）NaCl浓度下生长。KD-1 T在S 0（顶空：H2/CO2）和FeCl 3（顶空：N2）存在下厌氧生长。在好氧条件下，化能自养生长发生在S 0，黄铁矿，K2 S4 O 6，Na 2S 2 O3和H2。该菌株利用各种复杂的底物，如酵母提取物，但不以糖和氨基酸作为唯一碳源生长。核心脂质主要为钙基甘油-钙基胡萝卜素和钙基胡萝卜素。DNA G+C含量为30.6mol%。基于16 S rRNA和23 S rRNA基因的系统发育树分析表明，KD-1 T与大和硫化叶菌TA-1 T、东京硫化叶菌7 T和阳明硫化叶菌YM 1 T形成了一个独立的谱系。根据形态学、生理学、化学分类学和系统发育学分析结果，KD-1 T代表一新种，建议命名为Sulfurisphaera javensis sp. nov.。模式菌株为KD-1 T（=JCM 32117 T =InaCC Ar 81 T）。根据这些数据，我们还建议将Sulfolobus tokodaii Suzuki et al. 2002命名为Sulfurisphaera tokodaii comb. nov.（模式菌株7 T =JCM 10545 T =DSM 16993 T）。
A novel hyperthermophilic, acidophilic and facultatively anaerobic archaeon, strain KD-1T, was isolated from an acidic hot spring in Indonesia and characterized with the phylogenetically related species Sulfurisphaera ohwakuensis Kurosawa et al. 1998, Sulfolobus tokodaii Suzuki et al., 2002 and Sulfolobus yangmingensis Jan et al. 1999. Cells of KD-1T were irregular cocci with diameters of 0.9-1.3 µm. The strain grew at 60-90 °C (optimum 80-85 °C), pH 2.5-6.0 (optimum pH 3.5-4.0) and 0-1.0 % (w/v) NaCl concentration. KD-1T grew anaerobically in the presence of S0 (headspace: H2/CO2) and FeCl3 (headspace: N2). Under aerobic conditions, chemolithoautotrophic growth occurred on S0, pyrite, K2S4O6, Na2S2O3 and H2. This strain utilized various complex substrates, such as yeast extract, but did not grow on sugars and amino acids as the sole carbon source. The main core lipids were calditoglycerocaldarchaeol and caldarchaeol. The DNA G+C content was 30.6 mol%. Analyses of phylogenetic trees based on 16S rRNA and 23S rRNA genes indicated that KD-1T formed an independent lineage near Sulfurisphaera ohwakuensis TA-1T, Sulfolobus tokodaii 7T and Sulfolobus yangmingensis YM1T. On the basis of the results of morphological, physiological, chemotaxonomic and phylogenetic analyses, KD-1T represents a novel species of the genus Sulfurisphaera Kurosawa et al. 1998, for which the name Sulfurisphaera javensis sp. nov. is proposed. The type strain is KD-1T (=JCM 32117T=InaCC Ar81T). Based on the data, we also propose the reclassification of Sulfolobus tokodaii Suzuki et al., 2002 as Sulfurisphaera tokodaii comb. nov. (type strain 7T=JCM 10545T=DSM 16993T).