EB VIRUS-INDUCED B-LYMPHOCYTE CELL LINES PRODUCING SPECIFIC ANTIBODY
EB VIRUS-INDUCED B-LYMPHOCYTE CELL LINES PRODUCING SPECIFIC ANTIBODY
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DOI：
10.1038/269420a0
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发表时间：
1977-01-01
期刊：
影响因子：
64.8
通讯作者：
MAKEL, O
中科院分区：
文献类型：
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作者：
STEINITZ, M;KLEIN, G;MAKEL, O
EB病毒（EBV）是一种嗜淋巴细胞疱疹病毒，其将正常人B淋巴细胞转化为已建立的细胞系。这种“永生化”保留了原始B细胞的特征，包括EBV受体、补体受体、表面免疫球蛋白和分泌性免疫球蛋白。表面免疫球蛋白最常见的是IgM。EB病毒永生化株携带多个拷贝的病毒基因组，通过核酸杂交检测，并定期表达EB病毒特异性核抗原EBNA。已证实所有B细胞均携带EBV受体。如果是这样，并且如果所有的B细胞都能被EBV转化成细胞系，那么从能够产生针对适当抗原的特异性抗体的B淋巴细胞建立永久细胞系应该是可行的。显然，考虑到用转化病毒株如B 95 -8感染B淋巴细胞后获得的多克隆转化，有必要预先选择具有适当抗原结合位点的淋巴细胞。用抗原偶联的红细胞重置是用于这种预选的可想到的方法之一。虽然这将选择具有适当表面免疫球蛋白受体的淋巴细胞，但最近显示EBV转化激活正常淋巴细胞并诱导分泌性免疫球蛋白2的释放。如果这个过程是成功的，那么建立的细胞系可能会携带适当的表面受体，并分泌相应的抗体。这实际上是在这项研究中发现的，为建立产生特定抗体的细胞系开辟了道路。
EPSTEIN–BARR virus (EBV) is a lymphotropic herpesvirus that converts normal human B lymphocytes into established lines. This ‘immortalisation’ preserves the characteristics of the original B cell, including EBV receptors, complement receptors, surface immunoglobulin and secretory immunoglobulin. Surface immunoglobulin is most frequently IgM. EBV-immortalised lines carry multiple copies of the viral genome, detected by nucleic acid hybridisation, and regularly express an EBV-specific nuclear antigen, EBNA. It has been suggested1that all B-cells carry EBV receptors. If so, and if all B cells can be transformed into lines by EBV, it should be feasible to establish permanent lines from B lymphocytes capable of producing specific antibodies against appropriate antigens. Clearly, in view of the polyclonal transformation obtained after infection of B lymphocytes with a transforming virus strain, for example, B95-8, it will be necessary to pre-select lymphocytes with the appropriate antigen combining site. Resetting with antigen-coupled erythrocytes is one of the conceivable methods for such pre-selection. While this will select lymphocytes with the appropriate surface immunoglobulin receptors, it was recently shown that EBV-transformation activates normal lymphocytes and induces the release of secretory immunoglobulin2. If the procedure is successful, the established lines might therefore be expected to carry the appropriate surface receptor and also to secrete the corresponding antibody. This was actually found in this study, opening the way to the establishment of cell lines producing specific antibodies of choice.